| 引用本文: | 孙洞箫,周盾,邱振宇.血管紧张素Ⅱ对大鼠肾入球小动脉细胞内钙离子浓度的影响及Losartan的拮抗作用[J].中国现代应用药学,2010,27(10):857-860. |
| .Effect of Angiotensin II on Intracellular Free Calcium Concentration in RASMCs and Antagonism of Losartan[J].Chin J Mod Appl Pharm(中国现代应用药学),2010,27(10):857-860. |
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| 摘要: |
| 目的 探讨血管紧张素Ⅱ(AngⅡ)对肾小球入球小动脉细胞内钙离子浓度([Ca2+]i)的影响与高血压肾小动脉重建的关系及Losartan的治疗作用。方法 细胞培养：大鼠肾小球入球小动脉细胞随机分为4组。对照组：不加AngⅡ处理；AngⅡ组：加入终浓度为0.1 μmol·L-1的AngⅡ；Losartan组：加入终浓度为50 μmol·L-1的Losartan；AngⅡ+Losartan组：同时加入Losartan 50 μmol·L-1和AngⅡ0.1 μmol·L-1。负载后上机检测[Ca2+]i。结果 细胞培养显示AngⅡ引起了细胞收缩变化，表现为胞突变细变短，胞突回缩，胞体变圆，细胞长度与直径均明显缩小。Losartan处理组较AngⅡ组细胞形态变化减轻且Losartan对AngⅡ增高RASMCs内[Ca2+]i有明显抑制作用。结论 AngⅡ可引起肾小球入球小动脉细胞内[Ca2+]i的上升，导致细胞收缩，因此Ca2+超载可能是肾素-血管紧张素系统起作用的重要环节。Losartan可抑制细胞内Ca2+超载。 |
| 关键词: 肾小球入球小动脉细胞 高血压 血管紧张素Ⅱ Losartan 细胞内钙离子浓度 |
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| Effect of Angiotensin II on Intracellular Free Calcium Concentration in RASMCs and Antagonism of Losartan |
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SUN Dongxiao ZHOU Dun QIU Zhenyu
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| Abstract: |
OBJECTIVE To explore the effects of angiotensin(Ang Ⅱ) on intracellular free calcium concentration([Ca2+]i) in RASMCs and antagonism of Losartan. METHODS RASMCs were isolated and divided into four groups: control group, AngⅡ group, Losartan group and Ang Ⅱ+Losartan group. Confocal microscope was used with Fluo-3/AM as an indicator to detect the changes of [Ca2+]i. RESULTS Compared with control group, intracellular fluorescent intensity of RASMCs had no change in Losartan group, and was significantly higher in AngⅡ group (P<0.05). Value in AngⅡ+Losartan group was significantly lower than that in AngⅡ group (P<0.05). CONCLUSION AngⅡ may induce intracellular calcium overload in RASMCs, which can be significantly reduced by Losartan. |
| Key words: RASMCs hypertension angiotensinⅡ Losartan intracellular free calcium concentration |
