| 引用本文: | 余晓晖,夏鹏飞,李彤,刘峰林.红芪中红外光谱指纹图谱与其体外抗氧化活性的谱效相关性[J].中国现代应用药学,2016,33(8):1016-1020. |
| YU Xiaohui,XIA Pengfei,LI Tong,LIU Fenglin.Spectrum-effect Relationship Between Mid-infrared Spectrum Fingerprint of Hedysari Radix and Antioxidant Capacity in Vitro[J].Chin J Mod Appl Pharm(中国现代应用药学),2016,33(8):1016-1020. |
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| 摘要: |
| 目的 探讨红芪中红外光谱(mid-infrared spectrum，MIR)指纹图谱与抗氧化活性之间的谱效关系。方法 采用傅里叶变换红外光谱(FTIR)采集12个产地红芪药材的MIR指纹图谱，采用DPPH法测定12个产地红芪药材的抗氧化作用，采用Person相关性分析与多元非线性回归分析(逐步后退法)构建两者的谱效关系。结果 红芪煎煮液浓度为80 mg·mL-1时对DPPH的清除率最大；红芪MIR指纹图谱与抗氧化活性之间的谱效关系方程为Y=57.329X1-32.913X3+48.571X9(P<0.05，R=0.936)。结论 红芪MIR指纹图谱X1，X3 ，X9峰强度，即3 378，2 372，864 cm-1处吸收峰所代表的化学基团对其抗氧化活性有显著影响。 |
| 关键词: 红芪 中红外光谱指纹图谱 抗氧化 谱效关系 |
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| 基金项目:甘肃省自然科学基金(1308RJZA110) |
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| Spectrum-effect Relationship Between Mid-infrared Spectrum Fingerprint of Hedysari Radix and Antioxidant Capacity in Vitro |
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YU Xiaohui1,2, XIA Pengfei1,2, LI Tong1, LIU Fenglin1,2
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1.Gansu University of Traditional Chinese Medicine, Lanzhou 730000, China;2.Key Laboratory of Chemistry and Quality for Traditional Chinese Medicines of the College of Gansu Province, Gansu College of Traditional Chinese Medicine, Lanzhou 730000, China
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| Abstract: |
| OBJECTIVE To investigate the spectrum-effect relationship between mid-infrared spectrum(MIR) fingerprint of Hedysari Radix and antioxidant capacity in vitro. METHODS The MIR fingerprints of Hedysari Radix from 12 different regions were collected by FTIR, its antioxidative activity were determined by DPPH method, Person correlation analysis and multivariate nonlinear regression analysis (step-back method) were used to establish the relationship between MIR fingerprint of Hedysari Radix and antioxidant capacity in vitro. RESULTS The decocted solution of Hedysari Radix showed maximum clearance rate of DPPH at concentration of 80 mg·mL-1. The spectrum-effect relationship equation between MIR fingerprint of Hedysari Radix and antioxidant capacity was Y=57.329X1-32.913X3+48.571X9(P＜0.05, R=0.936). CONCLUSION There is close correlation between MIR fingerprint of Hedysari Radix and antioxidant capacity, and the absorption of 3 378, 2 372, 864 cm-1 had main effect on antioxidant capacity of Hedysari Radix. |
| Key words: Hedysari Radix mid-infrared spectrum fingerprint antioxidant spectrum-effect relationship |
