| 引用本文: | 马天成,贾英,罗洁,崔思娇,毕开顺.UPLC同时测定不同产地川芎中8种活性成分的含量[J].中国现代应用药学,2012,29(10):902-906. |
| MA Tiancheng,JIA Ying,LUO Jie,CUI Sijiao,BI Kaishun.Simultaneous Determination of Eight Active Components in Chuanxiong Rhizoma from Different Areas by UPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2012,29(10):902-906. |
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| 摘要: |
| 目的 建立同时测定不同产地川芎中咖啡酸、香草醛、阿魏酸、洋川芎内酯I、洋川芎内酯H、丁基苯酞、藁本内酯和丁烯基苯酞含量的UPLC方法。方法 采用ACQUITY UPLC，HSS T3色谱柱(2.1 mm×100 mm，1.8 μm)，流动相为甲醇-0.03%磷酸水溶液，梯度洗脱，PDA检测器，针对不同组分，选择其最大吸收波长作为检测波长。结果 咖啡酸、香草醛、阿魏酸、洋川芎内酯I、洋川芎内酯H、丁基苯酞、藁本内酯和丁烯基苯酞分别在0.082 8~3.312 μg·mL-1，0.047 85~ 1.914 μg·mL-1，4.420~176.8 μg·mL-1，2.035~81.4 μg·mL-1，0.470 4~18.82 μg·mL-1，1.044~41.75 μg·mL-1，13.55~542.0 μg·mL-1，1.785~71.40 μg·mL-1内呈良好的线性关系，平均回收率分别为101.0%，99.1%，98.9%，103.6%，96.3%，102.8%，98.1%，98.0%。结论 该方法简便、快捷、重复性好，可用于川芎药材及其制剂的质量控制。 |
| 关键词: 川芎 活性成分 UPLC |
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| 基金项目:辽宁省教育厅创新团队项目(2009T097) |
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| Simultaneous Determination of Eight Active Components in Chuanxiong Rhizoma from Different Areas by UPLC |
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MA Tiancheng1, JIA Ying1, LUO Jie1, CUI Sijiao1, BI Kaishun2
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1.Shenyang Pharmaceutical University, School of Traditional Chinese Materia Medica, Shenyang 110016, China;2.Shenyang Pharmaceutical University, School of Pharmacy, Shenyang 110016, China
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| Abstract: |
| OBJECTIVE To develop a UPLC method for determination of caffeic acid, vanillin, ferulic acid, senkyunolide I, senkyunolide H, butylphthalide, ligustilide and butylidenephthalide in Chuanxiong Rhizoma from different areas. METHODS The analysis was carried out on an HSS T3 (2.1 mm×100 mm, 1.8 μm) column. The mobile phase was composed of 0.03% phosphoric acid aqueous and methanol with gradient elution. The detection wavelengths were set at three different wavelengths according to different components. RESULTS The standard curves of eight active components showed a good linearity in 0.082 8-3.312 μg·mL-1, 0.047 85-1.914 μg·mL-1, 4.420-176.8 μg·mL-1, 2.035-81.4 μg·mL-1, 0.470 4-18.82 μg·mL-1, 1.044-41.75 μg·mL-1, 13.55-542.0 μg·mL-1, 1.785-71.40 μg·mL-1. The average recoveries were 101.0%, 99.1%, 98.9%, 103.6%, 96.3%, 102.8%, 98.1%, 98.0%, respectively. CONCLUSION This method is simple, quick, reproducible and can be used to control the quality of Chuanxiong Rhizoma and its preparations. |
| Key words: Chuanxiong Rhizoma active components UPLC |
