| 引用本文: | 黄超,梅梅,吕银丽,王向军.HPLC测定瑞替加滨的含量[J].中国现代应用药学,2012,29(10):950-952. |
| HUANG Chao,MEI Mei,Lü Yingli,WANG Xiangjun.Determination of Retigabine by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2012,29(10):950-952. |
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| 摘要: |
| 目的 建立HPLC测定瑞替加滨含量的方法。方法 色谱柱为Agilent ZORBAX SB-C18(150 mm×4.6 mm，3.5 μm)，流动相为庚烷磺酸钠溶液-乙腈，采用梯度洗脱，流速为1.0 mL·min-1，检测波长为250 nm，温度为30 ℃。结果 瑞替加滨在0.204~122.28 μg·mL-1内，峰面积与浓度呈良好的线性关系(r=0.999 9)，平均回收率99.9%，RSD=0.46%。结论 本法准确、重复性好，能与其同分异构体较好分离，适用于瑞替加滨的含量测定。 |
| 关键词: 高效液相色谱法 瑞替加滨 含量测定 |
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| Determination of Retigabine by HPLC |
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HUANG Chao1, MEI Mei1, Lü Yingli1, WANG Xiangjun2
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1.Zhejiang Jiuzhou Medicine Science and Technology Co., Ltd, Hangzhou 310051, China;2.School of Forestry and Bio-technology, Zhejiang A&F University, Lin’an 311300, China
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| Abstract: |
| OBJECTIVE To establish a method to determine the content of retigabine. METHODS Agilent ZORBAX SB-C18(150 mm×4.6 mm, 3.5 μm) was used as the analytical column. The mobile phase was a mixture of sodium heptanesulfonate solution-acetonitrile, and elute with linear gradient. The flow rate was 1.0 mL·min-1. Detection wavelength was 250 nm and column temperature was 30 ℃. RESULTS Retigabine has a good linearity in the range of 0.204-122.28 μg·mL-1 (r=0.999 9), the average recovery was 99.9% with RSD of 0.46%. CONCLUSION This method is accurate, repeatable and its isomers can be well separated. It can be applied to determine the content of retigabine. |
| Key words: HPLC retigabine content determination |
