| 引用本文: | 郭晓霞,李欣,王娟弟,李冬华,张明童,马潇,宋平顺.ICP-MS检测紫花地丁中37种无机元素含量[J].中国现代应用药学,2022,39(13):1731-1736. |
| GUO Xiaoxia,LI Xin,WANG Juandi,LI Donghua,ZHANG Mingtong,MA Xiao,SONG Pingshun.Content Determination of 37 Inorganic Element in Viola Yedoensis Makino by ICP-MS[J].Chin J Mod Appl Pharm(中国现代应用药学),2022,39(13):1731-1736. |
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| ICP-MS检测紫花地丁中37种无机元素含量 |
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郭晓霞1,2, 李欣1,2, 王娟弟1,2, 李冬华1,2, 张明童1,2, 马潇1,2, 宋平顺1,2
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1.甘肃省药品检验研究院, 兰州 730070;2.国家药品监督管理局中药材及饮片质量控制重点实验室, 兰州 730070
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| 摘要: |
| 目的 测定紫花地丁中37种无机元素的含量，并建立多元素的电感耦合等离子体质谱（inductively coupled plasma mass spectrometry，ICP-MS）测定方法，初步探索研究紫花地丁药材中重金属元素的污染情况。方法 以HNO3和H2O2作为消解溶剂，采用微波消解法前处理样品，通过ICP-MS同时测定紫花地丁中37种无机元素的含量。结果 37种无机元素在0.5~500 ng·mL-1内与峰面积呈良好的线性关系（r ≥ 0.99），在0.1，1和10 μg 3个添加水平下，大多数元素的平均回收率介于85.1%~120.6%，RSD（n=6）<6%，仪器精密度和重复性的RSD值均<3%；19批紫花地丁中5种中国药典2015年版明确需要控制的有害重金属元素（铜、铅、砷、汞、镉）的检出率分别为铜和铅100%，砷和汞89.5%和10.5%，镉在所有样品中均未检出；钼元素和锶元素在19批样品检出率均为100%，且含量远远高于其他元素，钼元素的最高含量为24.8 mg·kg-1；锶元素的含量则可高达67.6 mg·kg-1。结论 该方法具有简便、快速、准确的优点，可用于紫花地丁中37种无机元素的含量测定。 |
| 关键词: 紫花地丁 ICP-MS 无机元素 |
| DOI：10.13748/j.cnki.issn1007-7693.2022.13.011 |
| 分类号:R917 |
| 基金项目:中药材及饮片质量控制重点实验室项目（2020GSMPA-KL06-1，2020GSMPA-KL07，2020GSMPA-KL15）；甘肃省药品产业技术扶持项目（2019KF008）；甘肃省科学技术协会—2019年度甘肃海智计划特色示范项目；甘肃省2020年度重点人才项目（2020RCXM084） |
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| Content Determination of 37 Inorganic Element in Viola Yedoensis Makino by ICP-MS |
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GUO Xiaoxia1,2, LI Xin1,2, WANG Juandi1,2, LI Donghua1,2, ZHANG Mingtong1,2, MA Xiao1,2, SONG Pingshun1,2
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1.Gansu Institute for Drug Control, Lanzhou 730070, China;2.NMPA Key Laboratory for Quality Control of TCM, Lanzhou 730070, China
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| Abstract: |
| OBJECTIVE To determine the content of 37 kinds of inorganic element in Viola yedoensis Makino, and to establish the multi-element inductively coupled plasma mass spectrometry(ICP-MS) method to preliminary explore the pollution of heavy inorganic element in Viola yedoensis Makino. METHODS With HNO3 and H2O2 as digestion solvent, the samples were pretreated by microwave digestion method, and the contents of 37 kinds of inorganic element were determined simultaneously by ICP-MS method. RESULTS In the range of 0.5-500 ng·mL-1, 37 inorganic element showed good linear relationship(r ≥ 0.99). At three supplemental levels of 0.1, 1 and 10 μg, the average recoveries of most elements ranged from 85.1% to 120.6%, the RSDs(n=6) were <6%, and the RSDs of instrument precision and reproducibility were <3%; the detection rates of 5 kinds of harmful heavy inorganic element(Cu, Pb, As, Hg, Cd) that needed to be controlled in the Chinese Pharmacopoeia(2015 Edition) of 19 batches of Viola yedoensis Makino were as followed:the detection rates of Cu and Pb were 100%, As and Hg were 89.5% and 10.5%, respectively, and Cd was not detected in all the samples. The detection rates of molybdenum and strontium in 19 batches of samples were 100%, and there content were much higher than other elements. The highest content of molybdenum was 24.8 mg·kg-1. The content of strontium could be as high as 67.6 mg·kg-1. CONCLUSION The method has the advantages of simplicity, rapidness and accuracy. It can be used for the determination of 37 kinds of inorganic element in Viola yedoensis Makino. |
| Key words: Viola yedoensis Makino ICP-MS inorganic element |
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