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| 摘要: |
| 目的 建立痛经宁胶囊中芍药苷的HPLC含量测定方法。方法 采用Hypersil C18柱(250 mm×4.6 mm，5 μm)，以乙腈-0.1%磷酸(15∶85)为流动相；检测波长230 nm；流速：1.0 mL·min-1，柱温：30 ℃。结果 芍药苷在0.256~1.536 μg内线性关系良好，r=0.999 9，平均回收率为99.49%，RSD=0.56%(n=9)。结论 该方法简便、准确、重复性好、可作为痛经宁胶囊的含量测定方法。 |
| 关键词: 痛经宁胶囊 芍药苷 反相高效液相色谱法 |
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| Determination of Paeoniflorin in Tongjingning Capsules by RP-HPLC |
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JIANG Fan1 GUO Peiguo2 CAI Xiaojing1
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| Abstract: |
| OBJECTIVE To establish a method for determining the concentration of paeoniflorin in Tongjingning capsules by RP-HPLC. METHODS RP-HPLC was used. Chromatography conditions: Hypersil C18 column(250 mm×4.6 mm, 5 μm), the mobile phase consists of acetonitrile-0.1% phosphoric(15∶85), the flow rate was 1.0 mL·min-1, the detective wavelength was set at 230 nm, and column temperature was 30 ℃. RESULTS The calibration curve was linear in the range of 0.256-1.536 μg for paeoniflorin(r=0.999 9). The average recovery of paeoniflorin was 99.46% and RSD was 0.56% (n=9). CONCLUSION The method is simple and accurate with good repeatability, which can be used for the quality control of Tongjingning capsules. |
| Key words: Tongjingning capsules paeoniflorin RP-HPLC |
