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| 摘要: |
| 目的 建立HPLC测定利胆合剂中白藜芦醇含量的方法。方法 采用HYPERSIL ODS C18色谱柱(250 mm×4.6 mm，10 µm)；乙腈-水(25∶75)为流动相；流速为1 mL·min–1；检测波长为305 nm。结果 白藜芦醇在8.2~164 µg·mL–1内呈现良好的线性关系，r=0.999 7，重现性良好，平均回收率为87.02%，88.31%，90.67%。结论 本法检测快速，定量准确，可用于利胆合剂中白藜芦醇的含量测定。 |
| 关键词: 白藜芦醇 利胆合剂 HPLC |
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| Determination of Resveratrol in Longlianlidan Mixture by HPLC |
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SONG Jingxian
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| Abstract: |
| OBJECTIVE To establish an HPLC method for determination of resveratrol in Longlianlidan mixture. METHODS The determination of resveratrol in Longlianlidan mixture by HPLC was performed with HYPERSIL ODS C18 (250 mm×4.6 mm, 10 µm) and the mobile phase of acetonitrile-water(25∶75) at a flow rate of 1.0 mL·min–1, and the detection wavelength was conducted at 305 nm and at room temperature. RESULTS The calibration curve was linear within the range of 8.2-164 µg·mL–1 and the average recovery of resveratrol was 84.56%, 88.31%, 90.67%, respectively. CONCLUSION The HPLC method is precise, quick and suitable for determination of resveratrol in Longlianlidan mixtrue. |
| Key words: resveratrol Longlianlidan mixture HPLC |
