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| 摘要: |
| 目的 建立烫伤合剂中盐酸小檗碱的含量测定方法。方法 色谱柱为Inertsil C8-3柱(4.6 mm×250 mm，5 μm)，流动相为0.05 mol·L-1磷酸二氢钠溶液-乙腈-磷酸(70∶30∶0.08 )；流速为1 mL·min-1，柱温：35 ℃，检测波长为260 nm。结果 盐酸小檗碱在5.28~52.8 mg·L-1内峰面积与浓度呈良好的线性关系，Y=2.45×104X＋4.91×103(r=0.999 4)，平均回收率为100.30%，RSD为1.24% (n=6)。结论 方法简便、准确、重复性好，可作为控制产品质量的定量依据。 |
| 关键词: 烫伤合剂 盐酸小檗碱 高效液相色谱法 |
| DOI： |
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| Determination of Berberine Hydrochloride in Mixture for Burn and Scald by HPLC |
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XIAO Ping1 LI Wenshi2
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| Abstract: |
| OBJECTIVE To determine the content of berberine hydrochloride in mixture for burn and scald. METHODS The content of berberine hydrochloride in mixture for burn and scald was determined by HPLC with Inertsil C8-3 (4.6 mm× 250 mm, 5 μm) column. The mobile phase was 0.05 mol·L-1 sodium dihydrogen phosphate solution-acetonitrile-phosphoric (70∶30∶0.08) with a flow rate of 1 mL·min-1, the columm temperature was set at 35 ℃ and the wavelength of the detector was set at 260 nm. RESULTS The linear range was 5.28-52.8 mg·L-1 and the correlation cofficient was 0.999 4. The average recovery and the RSD were 100.30% and 1.24%(n=6). CONCLUSION The method is simple, accurate and repeatable. It is proved to be suitable for the quality control of mixture for burn and scald. |
| Key words: mixture for burn and scald berberine hydrochloride HPLC |
