HPLC测定雪胆胃肠丸中的延胡索乙素

许琼明<SUP>1</SUP>; 郭兆荣<SUP>1; 2</SUP>; 杨世林<SUP>1; 2; 3</SUP>; 李笑然<SUP>1*</SUP>; 高红伟<SUP>1</SUP>; 董更婷<SUP>1</SUP>; 王佳露<SUP>4</SUP>

引用本文:	许琼明,郭兆荣,杨世林,李笑然,高红伟,董更婷,王佳露.HPLC测定雪胆胃肠丸中的延胡索乙素[J].中国现代应用药学,2010,27(5):436-439.
	.Determination of Trahydorpalmatine in Xuedan Weichang Pill by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2010,27(5):436-439.

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HPLC测定雪胆胃肠丸中的延胡索乙素
许琼明,郭兆荣,杨世林,李笑然,高红伟,董更婷,王佳露

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摘要:

目的建立雪胆胃肠丸中延胡索乙素含量测定的方法。方法采用Hypersil ODS2柱 (250 mm×4.6 mm，5 μm)，乙腈-0.1%磷酸溶液(三乙胺调pH至6.0)(40∶60)为流动相；流速1.0 mL·min^-1；检测波长225 nm；柱温：25 ℃。结果延胡索乙素在4.8~48.0 μg·mL^-1内与其峰面积有良好的线性关系，其平均回收率为99.82%，RSD为1.00% (n=6)。结论所建方法简便易行、准确可靠，可用于雪胆胃肠丸中延胡索乙素的含量测定。

关键词: 雪胆胃肠丸延胡索乙素高效液相色谱法含量测定

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Determination of Trahydorpalmatine in Xuedan Weichang Pill by HPLC

XU Qiongming¹ GUO Zhaorong¹ 2 YANG Shilin¹ 2 3 LI Xiaoran^1* GAO Hongwei¹ DONG Gengting¹ WANG Jialu⁴

Abstract:

OBJECTIVE To establish an HPLC method for determination of contents of trahydorpalmatine in Xuedan Weichang pill. METHODS To utilize RP-HPLC, the Hypersil ODS2 column (250 mm×4.6 mm, 5 μm) was used with the mobile phase of acetonitrile-0.1% phosphate acid (adjustd to pH 6.0 by triethylamine) (60∶40). The flow rate was 1.0 mL·min^-1. The detection wavelength was 225 nm. The column temperature was 25 ℃. RESULTS The linear range of trahydorpalmatine was 4.8-48.0 μg·mL^-1. The average recoveries was 99.82% with RSD of 1.00% (n=6). CONCLUSION The method established is sensitive, accurate and can be used for quality control of Xuedan Weichang pill.

Key words: Xuedan Weichang pill trahydorpalmatine HPLC determination