腹膜透析用健肾解毒冻干粉剂中黄芪甲苷的含量测定

陈丹1; 王二丽2; 林奇泗1; 包国荣1; 刘永静1

引用本文:	陈丹1，王二丽2，林奇泗1，包国荣1，刘永静1.腹膜透析用健肾解毒冻干粉剂中黄芪甲苷的含量测定[J].中国现代应用药学,2009,(13):1143-1145.
	CHEN Dan1，WANG Erli2，LIN Qisi1，BAO Guorong1，LIU Yongjing1.Assay of Astragaloside Ⅳ in the Jianshenjiedu Lyophilized Powder for Peritoneal Dialysis[J].Chin J Mod Appl Pharm(中国现代应用药学),2009,(13):1143-1145.

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腹膜透析用健肾解毒冻干粉剂中黄芪甲苷的含量测定
陈丹1，王二丽2，林奇泗1，包国荣1，刘永静1

作者	单位
陈丹1，王二丽2，林奇泗1，包国荣1，刘永静1

摘要:

目的建立腹膜透析用健肾解毒冻干粉剂中黄芪甲苷的含量测定方法。方法采用反相高效液相色谱-蒸发光散射检测法，LiChroCART HPLC-Kartusche RP-18e（4.6 mm×250 mm，5 μm）色谱柱，流动相：乙腈-水（32∶68），流速：1.0 mL·min-1，检测器漂移管温度 100.6 ℃，氮气流速3.00 mL·min-1。结果方法中黄芪甲苷平均回收率为98.9%，RSD=1.19%（n=6）。结论建立了一种操作简便、准确性良好的高效液相色谱法控制腹膜用健肾解毒冻干粉剂中有效成分含量。

关键词: 腹膜透析用健肾解毒冻干粉剂黄芪甲苷反相高效液相色谱法蒸发光散射检测器

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Assay of Astragaloside Ⅳ in the Jianshenjiedu Lyophilized Powder for Peritoneal Dialysis

CHEN Dan1，WANG Erli2，LIN Qisi1，BAO Guorong1，LIU Yongjing1

Abstract:

OBJECTIVE To develop a RP-HPLC method for the assay of astragaloside Ⅳ in the Jianshenjiedu lyophilized powder for peritoneal dialysis(PDJSLP).METHODS The analytical chromatographic column was a LiChroCART HPLC-Kartusche RP-18e(4.6 mm×250 mm,5 μm)column.The mobile phase was acetonitrile-water(32∶68).The flow rater was 1.0 mL·min-1,and the detection shift temperature was 100.6 ℃.The N2 rater was 3.00 mL·min-1.RESULTS The average recovery of the method was 98.9%,RSD=1.19%(n=6).CONCLUSION The proposed method can be used in the quantitative analysis of astragaloside Ⅳ in the PDJSLP.And the method is convenient,economical and accurate with good reproducibility.

Key words: Jianshenjiedu lyophilized powder for peritoneal dialysis(PDJSLP) astragaloside Ⅳ RP-HPLC ELSD