| 引用本文: | 胡季强1，王如伟1，吕燊2，邱红英2.大孔树脂分离纯化延胡索总生物碱的工艺研究[J].中国现代应用药学,2009,(4):271-273. |
| HU Jiqiang1,WANG Ruwei1,LV Shen2,QIU Hongying2.Study on the Technology of Isolation and Purification of Gross Aalkaloid in Rhizoma Corydalis with Macropopous Adsorbing Resin[J].Chin J Mod Appl Pharm(中国现代应用药学),2009,(4):271-273. |
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| 摘要: |
| 目的研究大孔树脂分离纯化延胡索总生物碱的工艺条件及参数。方法以延胡索总生物碱提取率为评价指标筛选大孔树脂。结果经试验表明分离纯化的最佳工艺为D-101大孔树脂，上样量为延胡索药材与树脂体积比为1∶1（g·mL-1）；洗脱速度0.5 BV·h-1；去离子水洗脱杂质，洗脱体积为6 BV，80％乙醇洗脱总生物碱，洗脱体积为6 BV。结论该工艺简单可行，分离效果好，能满足于大生产的要求。 |
| 关键词: 大孔树脂 分离 纯化 延胡索 总生物碱 |
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| Study on the Technology of Isolation and Purification of Gross Aalkaloid in Rhizoma Corydalis with Macropopous Adsorbing Resin |
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HU Jiqiang1,WANG Ruwei1,LV Shen2,QIU Hongying2
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| Abstract: |
| OBJECTIVE To explore the purification method of gross alkaloid in Rhizoma Corydalis with macropopous adsorbing resin.METHODS The extracting yield of the gross alkaloid were taken as index to select the macroporous resin.RESULTS Optium conditions for isolation and purification were indentified, I.e. D-101 type macropopous adsorbing resin,adsorbing rate was 0.5 BV·h-1,water volume for removing impurities was 6 BV,concentration of the ethanol was 80%,and volume of ethanol was 6 BV.CONCLUSION The method is simple and feasible,with good separating effect which can meet industrial requirements. |
| Key words: macropopous adsorbing resin isolation purification Rhizoma Corydalis gross alkaloid |
