| 引用本文: | 丁逸梅，严相平，张晓蕾，张爱华.HPLC测定普瑞巴林含量[J].中国现代应用药学,2009,(3):243-245. |
| DING Yimei1,2,YAN Xiangping1,2,ZHANG Xiaolei3,ZHANG Aihua1,2.Determination of Pregabalin by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2009,(3):243-245. |
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| 摘要: |
| 目的建立普瑞巴林的含量测定方法。方法以2,4,6-三硝基苯磺酸作为衍生化试剂,采用柱前衍生化HPLC。色谱柱为C18柱,流动相为0.01mol·L-1乙酸铵水溶液(用磷酸调pH至4.0)-乙腈(43∶57),流速为1.0mL·min-1;柱温为40℃;检测波长为350nm。结果回收率99.18%,RSD=1.15%,普瑞巴林在3.320～49.80μg·mL-1内呈良好线性关系(r=0.9999)。最低定量限:0.5μg·mL-1。结论所建立的方法快速、简便、准确、灵敏,可用于普瑞巴林的含量测定。 |
| 关键词: 普瑞巴林 高效液相色谱法 含量测定 |
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| Determination of Pregabalin by HPLC |
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DING Yimei1,2,YAN Xiangping1,2,ZHANG Xiaolei3,ZHANG Aihua1,2
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| Abstract: |
| OBJECTIVE To establish an HPLC method for the determinaton of pregabalin. METHODSSamples were derivatized with 2,4,6-trinitrobenzene sulfonic acid (TNBSA). Separation was achieved on a reversed-phase C18 column with the mobile phase of 0.01 mol·L-1 ammonium acetate (pH=4.0)-acetonitrile (43∶57).The wavelength was 350 nm. RESULTSThe average recovery was 99.18%, RSD=1.15%.The calibration curve was linear between 3.320-49.80 μg·mL-1(r=0.999 9),the limit of quantification was 0.5 μg·mL-1 for pregabalin.CONCLUSIONThe method is simple, sensitive and praticable for the assay of pregabalin. |
| Key words: pregabalin HPLC determination |
