| 引用本文: | 尹进朝,李进,高永良.HPLC测定芹菜籽提取物中木犀草素含量[J].中国现代应用药学,2011,28(6):556-558. |
| YIN Jinchao,LI Jin,GAO Yongliang.Determination of Luteolin in Celery Seed Extract by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2011,28(6):556-558. |
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| 摘要: |
| 目的 建立高效液相色谱法测定芹菜籽提取物中木犀草素的含量。方法 采用Apollo C18柱(4.6 mm×250 mm，5 μm)；流动相为乙腈-0.1%磷酸溶液(30∶70)；流速为1.0 mL·min-1；进样量为20 μL；检测波长为269 nm；柱温为 30 ℃。结果 木犀草素浓度线性范围为0.50~10.0 μg·mL-1(r=0.999 8)，3个浓度的加样回收率分别为100.2%，100.1%和101.2%，RSD分别为1.02%，1.91%和1.75%。结论 本法操作简单、准确、专属性强，可作为芹菜籽提取物质量控制的方法。 |
| 关键词: 木犀草素 芹菜籽提取物 高效液相色谱法 含量测定 |
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| Determination of Luteolin in Celery Seed Extract by HPLC |
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YIN Jinchao1, LI Jin2, GAO Yongliang3
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1.Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;2.The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;3.Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China
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| Abstract: |
| OBJECTIVE To establish a method for the determination of luteolin in celery seed extract. METHODS The analysis was performed by HPLC with Apollo C18 column (4.6 mm×250 mm, 5 μm). The mobile phase was consisted of acetonitrile-0.1% phosphoric acid (30∶70), and the detection wavelength was set at 269 nm. The column temperature was 30 ℃. RESULTS The linear range was 0.50 to 10.0 μg·mL-1 for luteolin (r=0.999 8). The average recovery of three concentration were 100.2%, 100.1% and 101.2%, RSD were 1.02%, 1.91% and 1.75%. CONCLUSION The method is feasible, accurate and high selectivity for the determination of luteolin in celery seed extract. |
| Key words: luteolin ceelery seed extract HPLC content determination |
