| 引用本文: | 陈立江,段洪云,张胜,刘洋,刘宇.赤芍中芍药苷的分离纯化与结构鉴定[J].中国现代应用药学,2011,28(7):634-637. |
| CHEN Lijiang,DUAN Hongyun,ZHANG Sheng,LIU Yang,LIU Yu.Separation, Purification and Identification of Paeoniflorin from Paeonia Veitchii Lynch[J].Chin J Mod Appl Pharm(中国现代应用药学),2011,28(7):634-637. |
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| 摘要: |
| 目的 建立芍药苷的分离纯化与结构鉴定方法，有效提高芍药苷的含量。方法 将赤芍用醇水溶液加热回流提取，用AB-8大孔吸附树脂、制备液相等工序进行分离纯化，通过理化常数及光谱分析鉴定化合物结构，以HPLC测定其含量。结果 该法得到的芍药苷纯度为95.4%，总回收率为81.03%。结论 本工艺具有高效、简便、成本低、回收率高等优点，是一种良好的芍药苷分离纯化方法。 |
| 关键词: 赤芍 芍药苷 分离 纯化 AB-8大孔吸附树脂 |
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| Separation, Purification and Identification of Paeoniflorin from Paeonia Veitchii Lynch |
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CHEN Lijiang1, DUAN Hongyun1, ZHANG Sheng2, LIU Yang1, LIU Yu1
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1.College of Pharmacy, Liaoning University, Shenyang 110036, China;2.Liaoning Institute of Medicine, Shenyang 110015, China
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| Abstract: |
| OBJECTIVE To establish the method of separation, purifying and idenfication method of paeoniflorin, and to elevate effectually paeoniflorin content. METHODS Paeonia veitchii Lynch was extracted with ethanol-water solution, separated and purified by AB-8 macroporous resin and preparative HPLC. The structure was identified on the basis of physical and chemical characters and spectral analysis and quantitatively determined by HPLC. RESULTS Paeoniflorin with purity over 95.4%, can be prepared with the total recovery more than 81.03%. CONCLUSION High purity products can be obtained with this process which is high-performance, convenient, low cost and high recovery and is a good method of separation and purification of paeoniflorin. |
| Key words: Paeonia veitchii Lynch paeoniflorin isolation purification AB-8 macroporous resin |
