| 引用本文: | 吴明侠,崔永霞,王晶娟,张贵君.野菊花中总多糖提取条件的研究[J].中国现代应用药学,2011,28(2):133-136. |
| WU Mingxia,CUI Yongxia,WANG Jingjuan,ZHANG Guijun.Study on Extraction of Total Polysaccharides in Chrysanthemi Indici Flos[J].Chin J Mod Appl Pharm(中国现代应用药学),2011,28(2):133-136. |
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| 摘要: |
| 目的 研究野菊花总多糖的提取条件，并测定不同产地野菊花水煎剂中总多糖的含量。方法 采用苯酚-硫酸法测定总多糖含量，并对多糖显色方式进行优选。结果 野菊花中总多糖回收率为100.3%，RSD为1.19%，不同产地野菊花中总多糖含量存在差异。结论 该方法准确、快速、重复性好，可用于测定野菊花中总多糖的含量。 |
| 关键词: 野菊花 多糖 苯酚-硫酸法 |
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| 基金项目:河南中医学院博士科研基金(BSJJ2009-11) |
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| Study on Extraction of Total Polysaccharides in Chrysanthemi Indici Flos |
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WU Mingxia1, CUI Yongxia1, WANG Jingjuan2, ZHANG Guijun2
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1.Henan College of Traditional Chinese Medicine, Zhengzhou 450008, China;2.Beijing University of Chinese Medicine, Beijing 100102, China
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| Abstract: |
| OBJECTIVE To study on extraction of total polysaccharides in Chrysanthemi Indici Flos. And determine total polysaccharides in water extraction of Chrysanthemi Indici Flos from various habitats. METHODS Total polysaccharides were determined with phenol-sulfuric acid method and orthogonal test was used to select the best coloration. RESULTS Recovery of total polysaccharides was 100.3%, RSD was 1.19%, there was difference among Chrysanthemi Indici Flos from various habitats. CONCLUSION The method is accurate, repeatable and suitable to determine total polysaccharides in water extraction of Chrysanthemi Indici Flos. |
| Key words: Chrysanthemi Indici Flos polysaccharides phenol-sulfuric acid method |
