| 引用本文: | 王宇,朱碧楠.HPLC测定酮缬氨酸钙含量的方法优化[J].中国现代应用药学,2020,37(18):2233-2235. |
| WANG Yu,ZHU Binan.Improvement of Content Determination for α-Ketovaine Calcium by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2020,37(18):2233-2235. |
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| 摘要: |
| 目的 优化HPLC测定酮缬氨酸钙含量的方法。方法 HPLC采用CAPCELL PAK C18 MGⅡ柱（250 mmx4.6 mm，5 μm），流动相为高氯酸钠溶液（pH 2.5）-乙腈（96∶4），柱温为35℃，检测波长205 nm，流速1.0 mL·min-1。结果 酮缬氨酸钙在0.025~0.185 mg·mL-1内有良好的线性关系（r=1.000 0），平均回收率（n=9）为100.0%。结论 该方法简便、快速、准确，灵敏度高，重复性好，可用于酮缬氨酸钙的含量测定。 |
| 关键词: 酮缬氨酸钙 高效液相色谱法 C18柱 |
| DOI：10.13748/j.cnki.issn1007-7693.2020.18.011 |
| 分类号:R917.101 |
| 基金项目: |
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| Improvement of Content Determination for α-Ketovaine Calcium by HPLC |
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WANG Yu, ZHU Binan
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Zhejiang Institute for Food and Drug Control, Hangzhou 310052, China
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| Abstract: |
| OBJECTIVE To improve an HPLC method for the content determination of α-ketovaine calcium. METHODS The HPLC method was performed on CAPCELL PAK C18 MGⅡ column(250 mm×4.6 mm, 5 μm) with mobile phase of sodium perchlorate buffer(pH 2.5)-acetonitrile(96:4). Column temperature was 35℃, detective wavelength was 205 nm, and the flow rate was 1.0 mL·min-1. RESULTS The linear range of α-ketovaine calcium was 0.025-0.185 mg·mL-1 (r=1.000 0), the average recovery(n=9) was 100.0%. CONCLUSION The method is simple, rapid and accurate with good repeatability, which can be used for the determination of α-ketovaine calcium. |
| Key words: α-ketovaine calcium HPLC C18 column |
