| 引用本文: | 胡蔚,毛强.刺五加苷B对IL-1β诱导的骨关节炎软骨细胞凋亡的影响[J].中国现代应用药学,2020,37(21):2570-2575. |
| HU Wei,MAO Qiang.Effect of Acanthopanax B on IL-1β-induced Apoptosis of Osteoarthritic Chondrocytes[J].Chin J Mod Appl Pharm(中国现代应用药学),2020,37(21):2570-2575. |
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| 摘要: |
| 目的 探究刺五加苷B（acanthopanax B，ACA-B）对IL-1β诱导的骨关节炎软骨细胞凋亡的影响。方法 取SD大鼠软骨细胞随机分成5组，分别为对照组（PBS）、模型组（IL-1β）、ACA-B低剂量组（IL-1β+1×10-8 mol·L-1 ACA-B）、ACA-B中剂量组（IL-1β+1×10-7 mol·L-1 ACA-B）、ACA-B高剂量组（IL-1β+1×10-6 mol·L-1 ACA-B）；CCK8法检测不同浓度的ACA-B对软骨细胞增殖的影响，以及对IL-1β处理的软骨细胞活性的影响；采用Hoechst 33342染色观察软骨细胞核凋亡情况；采用qRT-PCR法检测凋亡相关基因的mRNA表达水平；采用Western blotting法检测凋亡相关蛋白的表达情况。结果 当ACA-B浓度为1×10-6 mol·L-1或1×10-7 mol·L-1时，能够显著促进软骨细胞增殖。与模型组相比，ACA-B各组细胞活力明显增加；Hoechst 33342染色结果显示，ACA-B能够明显改善IL-1β诱导的软骨细胞核碎裂，萎缩现象，抑制细胞凋亡；与模型组比较，ACA-B加药组细胞中Bax、Caspase-3、Caspase-8、Caspase-9的mRNA及蛋白水平显著降低，Bcl-2的mRNA及蛋白水平显著升高。结论 刺五加苷B能够有效抑制IL-1β诱导的骨关节炎软骨细胞凋亡。 |
| 关键词: 刺五加苷B IL-1β 骨关节炎 凋亡 |
| DOI：10.13748/j.cnki.issn1007-7693.2020.21.002 |
| 分类号:R965.1 |
| 基金项目:国家自然科学基金项目（81603639） |
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| Effect of Acanthopanax B on IL-1β-induced Apoptosis of Osteoarthritic Chondrocytes |
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HU Wei1, MAO Qiang2
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1.Hwa Mei Hospital, University of Chinese Academy of Science, Ningbo 315010, China;2.The First Affiliated Hospital of Zhejiang Chinese Medical University(Zhejiang Provincial Hospital of Traditional Chinese Medicine), Hangzhou 310006, China
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| Abstract: |
| OBJECTIVE To investigate the effect of acanthopanax B(ACA-B) on IL-1β-induced apoptosis of osteoarthritic chondrocytes. METHODS SD rat chondrocytes were randomly divided into 5 groups: control group (PBS), model group (IL-1β), ACA-B low-dose group (IL-1β+1×10-8 mol·L-1 ACA-B), ACA-B medium dose group (IL-1β+1×10-7 mol·L-1 ACA-B), and ACA-B high-dose group (IL-1β+1×10-6 mol·L-1 ACA-B); CCK8 assay was used to determine the effects of different concentrations of ACA-B on chondrocytes proliferation and IL-1β-treated chondrocytes activity; Hoechst 33342 staining was used to observe the apoptosis of cartilage nucleus; mRNA expression levels of apoptosis-related genes were detected by qRT-PCR; The expression of apoptosis-related proteins was detected by Western blotting. RESULTS ACA-B could significantly promote chondrocyte proliferation when the concentration was 1×10-6 mol·L-1 or 1×10-7 mol·L-1. Compared with model group, the cell activity of ACA-B groups were significantly increased. The results of Hoechst 33342 staining showed that ACA-B could significantly improve the nuclear fragmentation and atrophy of cartilage induced by IL-1β, and inhibit apoptosis. Compared with the model group, the mRNA and protein levels of Bax, Caspase-3, Caspase-8 and Caspase-9 in the ACA-B intervention group were significantly decreased, while the mRNA and protein levels of Bcl-2 were significantly increased. CONCLUSION ACA-B can effectively inhibit IL-1β-induced apoptosis of osteoarthritis chondrocytes. |
| Key words: acanthopanax B IL-1β osteoarthritis apoptosis |
