| 摘要: |
| 目的 探究枇杷叶提取物对哮喘小鼠的治疗效果。方法 60只BALB/c小鼠随机分为6组，空白组，模型组，枇杷叶提取物低、中、高剂量组（50，100，150 mg·kg-1），地塞米松组（0.5 mg·kg-1，阳性对照）。卵清蛋白（ovalbumin，OVA）诱导哮喘小鼠模型，各组小鼠分别灌胃给予相应药物。末次OVA造模后24 h吸入乙酰甲胆碱溶液检测小鼠气道高反应性。瑞氏-吉姆萨染色分类计数支气管肺泡灌洗液中炎症细胞数；ELISA检测Eotaxin、IL-4、IL-5、IL-13和OVA特异性IgE（OVA-specific IgE，OVA-sIgE）的水平；HE和AB-PAS染色观察小鼠肺组织的病理学改变；Western blotting检测肺组织中AKT、p-AKT、PI3K、Cyclin D1的蛋白表达。结果 枇杷叶提取物（100，150 mg·kg-1）能有效减少炎性细胞包括嗜酸性粒细胞、巨噬细胞、淋巴细胞、中性粒细胞的数量；降低Eotaxin、IL-4、IL-5、IL-13和OVA-sIgE的表达水平；抑制哮喘小鼠肺组织炎症细胞的浸润和黏液分泌；抑制哮喘模型中AKT、p-AKT、PI3K、Cyclin D1的蛋白表达。结论 枇杷叶提取物对OVA诱导的哮喘气道炎症有良好的抑制效果，可为枇杷叶治疗哮喘的研究提供参考。 |
| 关键词: 枇杷叶提取物|哮喘|气道炎症|气道重塑|Th2型细胞因子 |
| DOI：10.13748/j.cnki.issn1007-7693.2021.01.004 |
| 分类号:R285.5 |
| 基金项目:浙江省自然科学基金项目（LY15H270006） |
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| Effects of Loquat Leaf Extract on Airway Inflammation and Airway Remodeling Induced by Ovalbumin in Asthmatic Mice |
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HU Wei1, LI Lan2
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1.Hwa Mei Hospital, University of Chinese Academy of Science, Ningbo 315010, China;2.The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310006, China
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| Abstract: |
| OBJECTIVE To explore the therapeutic effect of Loquat leaf extract on asthma mice model. METHODS Sixty BALB/c mice were randomly divided into 6 groups, blank group, model group, Loquat leaf extract low, medium and high dose groups(50, 100, 150 mg·kg-1), dexamethasone group(0.5 mg·kg-1, positive control). Ovalbumin(OVA) induced asthma mouse model, each group of mice were gavaged with corresponding drugs. The airway hyperresponsiveness of mice was detected 24 h after the last OVA modelling. The number of inflammatory cells in bronchoalveolar lavage fluid was counted by Wright-Giemsa staining classification. The levels of Eotaxin, IL-4, IL-5, IL-13 and OVA-specific IgE(OVA-sIgE) were detected by ELISA. HE and AB-PAS staining were used to observe the pathological changes of mice lung tissue. Western blotting was used to detect the expression of AKT, p-AKT, PI3K and Cyclin D1 in lung tissue. RESULTS Loquat leaf extract(100, 150 mg·kg-1) could effectively reduce the number of inflammatory cells including eosinophils, macrophages, lymphocytes, and neutrophils; reduce the expression levels of Eotaxin, IL-4, IL-5, IL-13 and OVA-sIgE; inhibit the infiltration and mucus secretion of inflammatory cells in the lung tissue of asthmatic mice; inhibit the protein expression of AKT, p-AKT, PI3K and Cyclin D1 in asthma models. CONCLUSION Loquat leaf extract has a good inhibitory effect on OVA-induced airway inflammation in asthma, which can provide a reference for the study of Loquat leaf in the treatment of asthma. |
| Key words: Loquat leaf extract|asthma|airway inflammation|airway remodeling|Th2 cytokines |
