| 引用本文: | 海平,苏雅乐其其格,Narantsogt Natsagdorj,许良.HPLC对多叶棘豆中7个苷类化合物的定量分析[J].中国现代应用药学,2020,37(24):3005-3008. |
| HAI Ping,Suyalaqiqige,Narantsogt Natsagdorj,XU Liang.Quantitative Analysis of Seven Glycosides in Oxytropis Myriophylla by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2020,37(24):3005-3008. |
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| 摘要: |
| 目的 建立多叶棘豆中7个苷类化合物的定量分析方法。方法 以山奈酚-3-O-β-D-葡萄糖苷、异鼠李素-3-O-β-D-葡萄糖苷、（3R，4R）-4，7-二羟基-2'，3'-二甲氧基黄烷-4'-O-β-D-葡萄糖苷、3-羟基苯甲醇-4-O-β-D-葡萄糖苷、6-甲氧基香豆素-7-O-β-D-葡萄糖苷、2-羟基-6-甲氧基苯乙酮-4-O-β-D-葡萄糖苷和苯乙酮-4-O-β-D-葡萄糖苷为评价指标，采用HPLC对不同产地多叶棘豆进行定量分析。色谱柱：Topsil C18（250 mm×4.6 mm，5 μm）；流动相：乙腈-水，梯度洗脱；检测波长：261 nm；流速：1.0 mL·min-1；柱温为30℃；进样量为20 μL。结果 7个苷类化合物在一定范围内均具有较好的线性关系（r>0.999 2），其平均回收率分别为96.0%，97.0%，98.7%，96.0%，96.0%，97.7%，98.0%，RSD均<2.0%。被检测成分的含量变化分别为0.192~0.310，0.215~0.325，0.504~0.753，0.128~0.398，0.151~0.217，0.354~0.550，0.132~0.385 mg·g-1。结论 本实验所建立的含量测定方法可为综合评价该药材质量提供参考。 |
| 关键词: 高效液相色谱法 多叶棘豆 苷类化合物 同时测定 |
| DOI：10.13748/j.cnki.issn1007-7693.2020.24.011 |
| 分类号:R917.101 |
| 基金项目:国家民委-教育部蒙医药研发工程重点实验室开放课题项目（MDK2019068）；内蒙古自治区天然产物化学及功能分子合成重点实验室项目（MDK2016017） |
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| Quantitative Analysis of Seven Glycosides in Oxytropis Myriophylla by HPLC |
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HAI Ping1,2, Suyalaqiqige3, Narantsogt Natsagdorj2, XU Liang4
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1.College of Chemistry and Materials, Inner Mongolia University for Nationalities, Tongliao 028000, China;2.Department of Chemistry, School of Mathematic and Natural Science, Mongolian National University of Education, Ulaanbaatar 210648, Mongolia;3.College of Agronomy, Inner Mongolia University for Nationalities, Tongliao 028000, China;4.Graduate School, Inner Mongolia University for Nationalities, Tongliao 028000, China
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| Abstract: |
| OBJECTIVE To establish the quantitative analysis method for seven glycosides in Oxytropis myriophylla. METHODS Kaempferol-3-O-β-D-glucoside, isorhamnetin-3-O-β-D-glucoside, (3R,4R)-4,7-hydroxy-2',3'-dimethoxyisoflavane-4'-O-β- D-glucoside, 3-hydroxybenzyl alcohol-4-O-β-D-glucoside, 6-methoxycoumarin-7-O-β-D-glucoside, 2-hydroxy-6-methoxyacetophenone -4-O-β-D-glucoside and acetophenone-4-O-β-D-glucoside was taken as evaluation indexes, the quantitative analysis of Oxytropis myriophylla from different habitats was determined by HPLC. The chromatographic column: Topsil C18(250 mm×4.6 mm, 5 μm). The mobile phase was comprised of acetonitrile and water with a gradient elution. The detection wavelength was 261 nm with flow rate at 1.0 mL·min-1, column temperature at 30 ℃ and injection volume at 20 μL. RESULTS The 7 glycosides all had a good linearity within a certain range(r>0.999 2). The average recoveries were 96.0%, 97.0%, 98.7%, 96.0%, 96.0%, 97.7%, 98.0%, respectively, all of RSDs were<2.0%. Tested compounds and their content change in different samples were 0.192-0.310, 0.215-0.325, 0.504-0.753, 0.128-0.398, 0.151-0.217, 0.354-0.550, 0.132-0.385 mg·g-1, respectively. CONCLUSION The content detection method established in this experiment can provide reference for comprehensive evaluation of the quality of this medicinal material. |
| Key words: HPLC Oxytropis myriophylla glycosides simultaneous determination |
