| 引用本文: | 王伟,刘星雨,金辉辉,张东红,姚建标,宋永标,王如伟.GC同时测定麝香通心滴丸中龙脑、异龙脑和麝香酮的含量[J].中国现代应用药学,2020,37(11):1328-1332. |
| WANG Wei,LIU Xingyu,JIN Huihui,ZHANG Donghong,YAO Jianbiao,SONG Yongbiao,WANG Ruwei.Simultaneous Determination of Borneol, Isoborneol and Muscone in Shexiang Tongxin Dripping Pills by GC[J].Chin J Mod Appl Pharm(中国现代应用药学),2020,37(11):1328-1332. |
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| GC同时测定麝香通心滴丸中龙脑、异龙脑和麝香酮的含量 |
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王伟1, 刘星雨2, 金辉辉2, 张东红1, 姚建标2,3, 宋永标4, 王如伟1,2,3
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1.浙江中医药大学药学院, 杭州 310052;2.浙江康恩贝制药股份有限公司, 杭州 310052;3.浙江省中药制药技术重点实验室, 杭州 310052;4.内蒙古康恩贝药业有限公司圣龙分公司, 内蒙古 鄂尔多斯 017400
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| 摘要: |
| 目的 建立同时测定麝香通心滴丸中龙脑、异龙脑及麝香酮含量的气相色谱法。方法 采用HP-5MS毛细管色谱柱（30 m×0.32 mm，0.25 μm），检测器为FID检测器，检测器温度为250℃，进样口温度220℃。柱温以100℃为初始温度，保持2 min，以2℃·min-1升温至110℃，保持3 min，以50℃·min-1升温至205℃，保持7 min，以20℃·min-1升温至250℃，保持2 min；载气为氮气，流速为1 mL·min-1；进样方式为分流进样，分流比为10：1；进样量为1 μL，以萘为内标。结果 龙脑、异龙脑、麝香酮的浓度分别在0.054 68~0.437 4 mg·mL-1（r=0.999 9），0.070 67~0.565 4 mg·mL-1（r=0.999 9），0.004 512~0.036 10 mg·mL-1（r=0.999 7）内线性良好；平均回收率分别为97.91%（RSD=2.05%），100.79%（RSD=2.78%），105.36%（RSD=3.78%）。结论 该方法有效可靠，可用于麝香通心滴丸中龙脑、异龙脑和麝香酮的含量测定。 |
| 关键词: 气相色谱法 麝香通心滴丸 龙脑 异龙脑 麝香酮 |
| DOI：10.13748/j.cnki.issn1007-7693.2020.11.009 |
| 分类号:R917.101 |
| 基金项目: |
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| Simultaneous Determination of Borneol, Isoborneol and Muscone in Shexiang Tongxin Dripping Pills by GC |
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WANG Wei1, LIU Xingyu2, JIN Huihui2, ZHANG Donghong1, YAO Jianbiao2,3, SONG Yongbiao4, WANG Ruwei1,2,3
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1.Pharmacy College of Zhejiang Chinese Medical University, Hangzhou 310052, China;2.Zhejiang Conba Pharmaceutical Co., Ltd., Hangzhou 310052, China;3.Zhejiang Provincial Key Laboratory of Traditional Chinese Medicine Pharmaceutical Technology, Hangzhou 310052, China;4.Inner Mongolia Conba Pharmaceutical Co., Ltd., Shenglong Branch, Erdos 017400, China
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| Abstract: |
| OBJECTIVE To establish a GC method for the simultaneous determination of borneol, isoborneol and muscone in Shexiang Tongxin Dripping Pills. METHODS Sample of Shexiang Tongxin Dripping pills were determined by HP-5MS capillary column(30 m×0.32 mm, 0.25 μm). FID was used as detector with a temperature of 250℃, and the inlet temperature was 220℃. The column temperature was programmed to start at 100℃ maintained for 2 min, rised to 110℃ at a rate of 2℃·min-1, maintained for 3 min, and then rised to 205℃ at a rate of 50℃·min-1, maintained for 7 min, and then rised to 250℃ at a rate of 20℃·min-1 maintained for 2 min. Nitrogen(1 mL·min-1) was used as the carrying gas, and the split ratio was 10:1. The injection volume was 1 μL and naphthalene was used as internal standard. RESULTS The linear ranges of borneol, isoborneol and muscone were 0.054 68-0.437 4 mg·mL-1(r=0.999 9), 0.070 67-0.565 4 mg·mL-1(r=0.999 9), 0.004 512-0.036 10 mg·mL-1(r=0.999 7), respectively. The average recovery rates(n=6) were 97.91%(RSD=2.05%), 100.79% (RSD=2.78%), 105.36%(RSD=3.78%), respectively. CONCLUSION This method is reliable and accurate, which can be used for the determination of borneol, isoborneol and muscone in Shexiang Tongxin Dripping Pills. |
| Key words: GC Shexiang Tongxin Dripping pills borneol isoborneol muscone |
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