| 引用本文: | 陈洋洋,李梦媛,李雪莹,王琪,郝若祎,阎雪莹.染料木素MePEG-PLGA共聚物胶束体内外抗肿瘤作用[J].中国现代应用药学,2020,37(12):1442-1447. |
| CHEN Yangyang,LI Mengyuan,LI Xueying,Wang Qi,HAO Ruoyi,YAN Xueying.Anti-tumor Effect of Genistein MePEG-PLGA Copolymer Micelles in vitro and in vivo[J].Chin J Mod Appl Pharm(中国现代应用药学),2020,37(12):1442-1447. |
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| 摘要: |
| 目的 探讨染料木素MePEG-PLGA共聚物胶束对人肝癌SMMC-7721细胞的体内外抗肿瘤作用。方法 采用MTT法、Annexin V-FITC/PI染法分别考察染料木素MePEG-PLGA共聚物胶束对人肝癌SMMC-7721细胞的细胞毒性、细胞凋亡、细胞周期的影响；通过荷肝SMMC-7721瘤裸鼠体内抗肿瘤试验，考察染料木素胶束的体内抗肿瘤效应。结果 染料木素胶束对肝癌SMMC-7721细胞的抑制率均高于染料木素，最高抑制率分别可达83.26%，78.25%。流式细胞仪检测结果显示，染料木素胶束组能够提高染料木素对SMMC-7721细胞凋亡的诱导能力，并且呈浓度依赖性。5，10，20 μg·mL-1的染料木素组和染料木素胶束组药物作用于SMMC-7721细胞48 h时，染料木素胶束能够明显提高染料木素对细胞G2/M期细胞的阻滞作用，并且呈现浓度依赖性。体内抗肿瘤试验结果表明，相同浓度的染料木素胶束组的抑瘤效果显著强于染料木素乳剂组，呈浓度依赖性，最高抑瘤率可达55.41%。结论 染料木素MePEG-PLGA共聚物胶束在体内和体外均有显著的抗肝癌作用。 |
| 关键词: 染料木素 MePEG-PLGA共聚物胶束 细胞凋亡 抗肿瘤 |
| DOI：10.13748/j.cnki.issn1007-7693.2020.12.007 |
| 分类号:R965.1 |
| 基金项目:黑龙江省自然科学基金项目（ZD2016019） |
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| Anti-tumor Effect of Genistein MePEG-PLGA Copolymer Micelles in vitro and in vivo |
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CHEN Yangyang, LI Mengyuan, LI Xueying, Wang Qi, HAO Ruoyi, YAN Xueying
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College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China
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| Abstract: |
| OBJECTIVE To study the anti-tumor effect of genistein(GEN) MePEG-PLGA copolymer micelles on human hepatoma SMMC-7721 cells in vitro and in vivo. METHODS MTT assay, Annexin V-FITC/PI staining were used to investigate the effects of GEN MePEG-PLGA copolymer micelles on cytotoxicity, apoptosis and cell cycle of human hepatoma SMMC-7721 cells. The in vivo anti-tumor effect of GEN micelles was investigated in nude mice bearing hepatocellular carcinoma SMMC-7721 tumor in vivo. RESULTS The inhibitory rate of GEN micelles in SMMC-7721 cells were higher than GEN and the highest inhibition rates were 83.26% and 78.25%, respectively. The results of flow cytometry showed that the GEN micelles group could increase the ability of GEN to induce apoptosis of SMMC-7721 cells and presented a concentrationdependent manner. When the concentration of 5, 10, 20 μg·mL-1 GEN and GEN micelles groups acted on SMMC-7721 cells for 48 h, the GEN micelles could significantly enhance the ability of arrest cell in G2/M phase for GEN, and showing a concentration-dependent manner. In vivo anti-solid tumor experimental results showed that the same concentration of GEN micelles group had significantly better anti-tumor effect than GEN emulsion, and in a concentration-dependent manner, the highest tumor inhibition rate was 55.41%. CONCLUSION The GEN MePEG-PLGA copolymer micelles has significant anti-hepatocarcinoma effects both in vivo and in vitro. |
| Key words: genistein MePEG-PLGA copolymer micelles apoptosis antitumor |
