| 引用本文: | 胡轲,李珊珊,刘炜,罗凯文,邢亚东,李娴.双波长HPLC同时测定生地黄中6种化合物的含量[J].中国现代应用药学,2020,37(12):1478-1482. |
| HU Ke,LI Shanshan,LIU Wei,LUO Kaiwen,XING Yadong,LI Xian.Simultaneous determination of 6 compounds in Rehmanniae Radix by dual-wavelength HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2020,37(12):1478-1482. |
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| 摘要: |
| 目的 采用双波长HPLC同时测定生地黄中6种化合物（梓醇、地黄苷D、地黄苷A、益母草苷、毛蕊花糖苷、异类叶升麻苷）的含量。方法 菲罗门Neptune C18色谱柱（4.6 mm×250 mm，5μm）；流动相：乙腈-0.1%磷酸溶液，梯度洗脱；流速：1.0 mL·min-1；柱温30℃；检测波长203 nm（梓醇、地黄苷D、地黄苷A、益母草苷），334 nm（毛蕊花糖苷、异类叶升麻苷）。结果 6种化合物在各自的范围内呈现良好的线性关系（r>0.9990），3个浓度水平回收率96.33%~98.55%，RSD为0.97%~1.45%。结论 该法简单快捷、稳定可靠，准确率高，重复性好，可用于生地黄质量评价。 |
| 关键词: 生地黄 高效液相色谱法 梓醇 地黄苷D 地黄苷A 益母草苷 毛蕊花糖苷 异类叶升麻苷 |
| DOI：10.13748/j.cnki.issn1007-7693.2020.12.014 |
| 分类号:R917.101 |
| 基金项目:安徽省自然科学基金项目（1908085QH373，1908085QH376）；蚌埠医学院自然科学基金面上项目（BYKY1873）；蚌埠医学院科技发展基金项目（BYKF1816，BYKF1725） |
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| Simultaneous determination of 6 compounds in Rehmanniae Radix by dual-wavelength HPLC |
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HU Ke1, LI Shanshan2, LIU Wei1, LUO Kaiwen2, XING Yadong2, LI Xian2
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1.Taihe County Market Supervision and Inspection Institute, Fuyang 236600, China;2.Bengbu Medical College, Bengbu 233000, China
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| Abstract: |
| OBJECTIVE To establish a dual-wave length HPLC method for the determination of 6 components in Rehmanniae Radix (including catalpol, rehmannioside D, rehmannioside A, leonuride, acteoside and isoacteoside). METHODS Neptune C18 column(4.6 mm×250 mm, 5 μm) was selected for separation with acetonitrile-0.1% phosphoric acid solution as the mobile phase for gradient elution, the flow rate was 1.0 mL·min-1, the column temperature was maintained at 30 ℃ and the detection wavelength was set at 203 nm for catalpol, rehmannioside D, rehmannioside A and leonuride, 334 nm for acteoside and isoacteoside. RESULTS Six components showed good linear relationships(r>0.9990) in their own ranges. The average recoveries at three levels ranged from 96.33% to 98.55% with RSD of 0.97% to 1.45%. CONCLUSION This method is simple, accurate, reliable, stable and reproducible, which can be used for the quality control of Rehmanniae Radix. |
| Key words: Rehmanniae Radix HPLC catalpol rehmannioside D rehmannioside A leonuride acteoside isoacteoside |
