| 引用本文: | 周黎琴,石苏英.人参与五灵脂配伍对人参皂苷含量的影响[J].中国现代应用药学,2020,37(12):1474-1477. |
| ZHOU Liqin,SHI Suying.Effects of Compatibility Between Panax Ginseng and Trogopterus Dung on the Ginsenosides Content[J].Chin J Mod Appl Pharm(中国现代应用药学),2020,37(12):1474-1477. |
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| 摘要: |
| 目的 通过测定人参、红参、生晒参与五灵脂以不同比例配伍后人参皂苷的含量，探讨"人参畏五灵脂"的作用机制。方法 采用Agilent Athena C18色谱柱（4.6 mm×250 mm，5 μm），以乙腈（A）-磷酸盐缓冲液（B，pH 6.8）为流动相，进行梯度洗脱，流速1.0 mL·min-1，柱温25℃，检测波长203 nm。结果 人参皂苷Rg1、Re、Rf、Rb1、Rc、Rb2、Rd进样量分别在0.03 6 2~1.16 μg（r=0.998 5）、0.037 1~1.19 μg（r=0.999 0）、0.030 3~0.97 μg（r=0.991 4）、0.035 0~1.12 μg（r=0.993 9）、0.030 6~0.98 μg（r=0.997 3）、0.030 3~0.97 μg（r=0.999 1）、0.031 2~1.00 μg（r=0.999 1）内呈良好的线性关系；加样回收率RSD均<3%，方法的稳定性、重复性和精密度均良好（RSD<3%）。结论 人参、红参、生晒参与五灵脂配伍后7种人参皂苷含量显著下降，不同炮制方法的人参五灵脂配伍后，在比例为1：4时，人参皂苷Rb1、Rb2下降最大，达到90%，人参皂苷Rg1含量减少最小，但也下降了70%以上。 |
| 关键词: 人参 红参 生晒参 五灵脂 人参皂苷 高效液相色谱法 |
| DOI：10.13748/j.cnki.issn1007-7693.2020.12.013 |
| 分类号:R284.1 |
| 基金项目:浙江省中医药科技计划项目（2015ZB122） |
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| Effects of Compatibility Between Panax Ginseng and Trogopterus Dung on the Ginsenosides Content |
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ZHOU Liqin, SHI Suying
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Zhuji People's Hospital, Zhuji 311800, China
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| Abstract: |
| OBJECTIVE To investigate the action mechanism by detecting the ginsenoside content changes in panax genseng, red ginseng, white ginseng with different proportions of trogopterus dung. METHODS The separation was performed on Agilent Athena C18(4.6 mm×250 mm, 5 μm) column and the mobile phase consisted of acetonitrile(A) and H3PO4(B, pH6.8) water solution in gradient elution at flow rate of 1.0 mL·min-1, the column temperature was 25℃, the detection wavelength was set at 203 nm. RESULTS The linear ranges were 0.036 2-1.16 μg(r=0.998 5) for Rg1, 0.037 1-1.19 μg(r=0.999 0) for Re, 0.030 3-0.97 μg(r=0.991 4) for Rf, 0.035 0-1.12 μg(r=0.993 9) for Rb1, 0.030 6-0.98 μg(r=0.997 3) for Rc, 0.030 3-0.97 μg (r=0.999 1) for Rb2, 0.031 2-1.00 μg(r=0.999 1) for Rd. The sample recovery rate RSD <3%, the stability, repeatability and precision of the method were good(RSD <3%). CONCLUSION The contents of seven ginsenoside decreased significantly when panax ginseng, red ginseng, white ginseng combined with trogopterus dung, and when the ratio was 1:4, Rb1 and Rb2 decreased the maximum, highest fall achieve 90%. The content of Rg1 reduced the minimum, but also fell more than 70%. |
| Key words: panax ginseng red ginseng white ginseng trogopterus dung ginsenosides HPLC |
