| 引用本文: | 赵金龙,黄和涛,梁桂洪,曾令烽,吴明,林炯同,韩燕鸿,刘军.基于网络药理学的巴戟天治疗骨质疏松的通路及靶标[J].中国现代应用药学,2020,37(11):1301-1308. |
| ZHAO Jinlong,HUANG Hetao,LIANG Guihong,ZENG Lingfeng,WU Ming,LIN Jiongtong,HAN Yanhong,LIU Jun.Pathway and Target of Morindae Officinalis Radix in the Treatment of Osteoporosis Based on Network Pharmacology[J].Chin J Mod Appl Pharm(中国现代应用药学),2020,37(11):1301-1308. |
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| 基于网络药理学的巴戟天治疗骨质疏松的通路及靶标 |
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赵金龙1,2, 黄和涛1,2, 梁桂洪2,3, 曾令烽2,3, 吴明1,2, 林炯同1,2, 韩燕鸿1,2, 刘军2,3
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1.广州中医药大学第二临床医学院, 广州 510405;2.广东省中医药科学院, 骨与关节退变及损伤研究团队, 广州 510120;3.广州中医药大学第二附属医院, 广东省中医院, 广州 510120
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| 摘要: |
| 目的 通过网络药理学方法探讨巴戟天治疗骨质疏松症的作用机制及原理。方法 通过中药系统药理学分析平台数据库提取巴戟天中的活性成分和有关的靶标蛋白。运用可视化软件Cytoscape 3.5.1构建巴戟天有效成分与靶标蛋白关系网络进行拓扑学分析。使用STRING10.5数据库进行蛋白之间相互作用网络的构建与数据分析。借助David v6.8数据库进行生物学信号通路功能富集分析。结果 巴戟天的核心活性化合物有β-谷甾醇、2-羟基-1，5-二甲氧基-6-（甲氧基甲基）-9，10-蒽二酮、茜素-2-甲醚等；关键的靶标有前列腺素G/H合成酶2、热休克蛋白HSP 90、孕酮受体等。巴戟天有效活性成分主要作用于疾病的关键蛋白主要有ESR1、JUN、CASP3、PGR、CASP8等。KEGG结果表明巴戟天主要作用于Estrogen signaling pathway、PI3K-Akt signaling pathway等信号通路。结论 巴戟天主要通过多种途径、多种信号通路作用于多种靶点发挥抗骨质疏松症的作用，其功效可能与蒽醌类化合物、cGMP-PKG signaling pathway等信号通路以及ESR1、JUN等靶标蛋白相关。 |
| 关键词: 巴戟天 骨质疏松 网络药理学 通路 机制 原理 网络 |
| DOI：10.13748/j.cnki.issn1007-7693.2020.11.004 |
| 分类号:R285.6 |
| 基金项目:广东省财政厅项目（[2014]157，[2018]8）；广东省中医药管理局项目（20201129）；广东省中医院中医药科学技术研究专项（YN2019ML08，YK2013B2N19，YN2015MS15）；广东省医学科学技术研究基金项目（A2020105，B2019091）；广东省普通高校重点科研平台和科研项目（2018KQNCX041） |
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| Pathway and Target of Morindae Officinalis Radix in the Treatment of Osteoporosis Based on Network Pharmacology |
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ZHAO Jinlong1,2, HUANG Hetao1,2, LIANG Guihong2,3, ZENG Lingfeng2,3, WU Ming1,2, LIN Jiongtong1,2, HAN Yanhong1,2, LIU Jun2,3
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1.Second Clinical Medical College of Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China;2.Guangdong Academy of Traditional Chinese Medicine, Research Team on Bone and Joint Degeneration and Injury, Guangzhou 510120, China;3.Second Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangdong Province Hospital of Traditional Chinese Medicine, Guangzhou 510120, China
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| Abstract: |
| OBJECTIVE To explore the mechanism and principle of Morindae Officinalis Radix in the treatment of osteoporosis based on network pharmacology. METHODS The active ingredients and related target proteins of Morindae Officinalis Radix were extracted from TCMSP. The relationship network between active components and target proteins of Morindae Officinalis Radix was constructed by using the visualization software Cytoscape 3.5.1 for topological analysis. And the STRING 10.5 was used to construct protein-protein interaction network and analyze data. The enrichment of biological signaling pathway function was analyzed by using David v6.8. RESULTS The core compounds of Morindae Officinalis Radix were beta-sitosterol, 2-hydroxy-1,5-dimethoxy-6-(methoxymethyl)-9,10-anthraquinone, alizarin-2-methylether, and other key targets were prostaglandin G/H synthase 2, heat shock protein 90, progesterone receptor, etc. The active components of Morindae Officinalis Radix mainly acted on the key proteins, such as ESR1, JUN,CASP3, PGR, CASP8, etc. KEGG results showed that Morindae Officinalis Radix acted on Estrogen signaling pathway, PI3K-Akt signaling pathway and other signaling pathways. CONCLUSION Morindae Officinalis Radix plays an anti-osteoporosis role mainly through a variety of pathways and signaling pathways acting on a variety of targets. At the same time, it is predicted that the anti-osteoporosis effect of Morindae Officinalis Radix may be related to anthraquinone compounds, cGMP-PKG signaling pathways and target proteins such as ESR1, JUN, etc. |
| Key words: Morindae Officinalis Radix osteoporosis network pharmacology pathway mechanism principle network |
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