| 引用本文: | 杨东方,姚雪峰,胡云飞.HPLC同时测定不同产地密蒙花中4种化合物的含量[J].中国现代应用药学,2020,37(8):921-925. |
| YANG Dongfang,YAO Xuefeng,HU Yunfei.Simultaneous Determination of 4 Components in Buddleja Officinalis Maxim. from Different Regions by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2020,37(8):921-925. |
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| 摘要: |
| 目的 采用HPLC同时测定密蒙花中4种化合物（毛蕊花糖苷、蒙花苷、芹菜素、木犀草素）的含量，并对不同产地的密蒙花进行比较。方法 采用Waters SunfireTM C18色谱柱（4.6 mm×250 mm，5μm），以乙腈-0.2%磷酸水溶液为流动相，梯度洗脱；流速1.0 mL·min-1；柱温30℃；检测波长330 nm（毛蕊花糖苷、蒙花苷、芹菜素）和350 nm（木犀草素）。结果 4种化合物在各自的范围内呈现良好的线性关系（r>0.999 0），平均回收率为97.75%~98.49%，RSD为0.7%~1.5%。测得不同产地毛蕊花糖苷、蒙花苷、芹菜素、木犀草素的含量分别为21.046 3~42.886 3，8.514 4~17.400 8，0.317 3~1.205 3，0.030 4~0.145 6 mg·g-1。结论 该法稳定可靠，重复性好，可为密蒙花质量评价提供一定依据。 |
| 关键词: 密蒙花 高效液相色谱法 毛蕊花糖苷 蒙花苷 芹菜素 木犀草素 |
| DOI：10.13748/j.cnki.issn1007-7693.2020.08.005 |
| 分类号:R284.1;R917.101 |
| 基金项目:国家中医药管理局全国中药特色技术传承人才培训项目（国中医药人教函[2019]43号）；国家质量监督检验检疫总局科技计划项目（2015QK098）；山西省高等学校科技创新项目（20171118） |
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| Simultaneous Determination of 4 Components in Buddleja Officinalis Maxim. from Different Regions by HPLC |
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YANG Dongfang1, YAO Xuefeng1, HU Yunfei2,3
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1.Shanxi Pharmaceutical Vocational College, Taiyuan 030031, China;2.Bozhou University, Bozhou 236800, China;3.National Chinese Medicinal Materials Product Quality Supervision and Inspection Center, Bozhou 236800, China
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| Abstract: |
| OBJECTIVE To establish an HPLC method for simultaneous determination of acteoside, linarin, apigenin, luteolin in Buddleja officinalis Maxim. and to compare the content of Buddleja officinalis Maxim. from different origins. METHODS The analysis was performed on Waters SunfireTM C18 analytical column(4.6 mm×250 mm, 5 μm) with the gradientelution of acetonitrile-0.05% phosphoric acid solution at a flow rate of 1.0 mL·min-1, the column temperature was 30℃ and the detection wavelength was set at 330 nm(acteoside, linarin, apigenin) and 350 nm(luteolin). RESULTS Four constituents showed good linear relationships(r>0.999 0) within their own ranges, whose average recoveries were 97.75%-98.49% with the RSDs of 0.7%-1.5%. The contents of acteoside, linarin, apigenin, luteolin in samples of different regions were in ranges of 21.046 3-42.886 3, 8.514 4-17.400 8, 0.317 3-1.205 3, 0.030 4-0.145 6, respectively. CONCLUSION This reliable, stable and reproducible method can be used to evaluate the quality of Buddleja officinalis Maxim.. |
| Key words: Buddleja officinalis Maxim. HPLC acteoside linarin apigenin luteolin |
