| 引用本文: | 赵振霞,王晓蕾,雷蓉,王敏,刘永利.UPLC-MS/MS检测乌鸡白凤丸中非法添加西洋参[J].中国现代应用药学,2020,37(4):420-424. |
| ZHAO Zhenxia,WANG Xiaolei,LEI Rong,WANG Min,LIU Yongli.Determination of Panax Quinquefolium Illegally Added in Wuji Baifeng Pills by UPLC-MS/MS[J].Chin J Mod Appl Pharm(中国现代应用药学),2020,37(4):420-424. |
|
| 摘要: |
| 目的 建立UPLC-MS/MS测定乌鸡白凤丸中非法添加的西洋参。方法 使用Waters ACQUITY BEH C18色谱柱（2.1 mm×100 mm，1.7 μm），以乙腈和水为流动相，梯度洗脱，流速为0.35mL·min-1，柱温40℃，电喷雾电离源（ESI-），多反应离子监测（MRM）扫描方式进行检测。结果 拟人参皂苷F11在0.1130~451.9 ng·mL-1范围内线性关系良好（r=0.999），重复性的相对标准偏差为3.5%，拟人参皂苷F11平均加样回收率（n=9）为96.9%，检出限为0.1 ng·mL-1，定量限为0.4 ng·mL-1。结论 所建立的方法快速准确、灵敏度高，可用于乌鸡白凤丸中非法添加西洋参的筛选和检测。 |
| 关键词: 超高效液相色谱-串联质谱法 乌鸡白凤丸 西洋参 拟人参皂苷F11 非法添加 |
| DOI：10.13748/j.cnki.issn1007-7693.2020.04.007 |
| 分类号:R917 |
| 基金项目:“重大新药创制”国家科技重大专项（2014ZX09304307-002） |
|
| Determination of Panax Quinquefolium Illegally Added in Wuji Baifeng Pills by UPLC-MS/MS |
|
ZHAO Zhenxia, WANG Xiaolei, LEI Rong, WANG Min, LIU Yongli
|
|
Hebei Institute for Drug Control, Shijiazhuang 050011, China
|
| Abstract: |
| OBJECTIVE To establish adetection method for Panax quinquefolium a illegally added in Wuji Baifeng pills by UPLC-MS/MS. METHODS The analysis was performed on Waters ACQUITY BEH C18 column(2.1 mm×100 mm, 1.7 μm), with acetonitrile-water as the mobile phase with gradient elution and the flow rate of 0.35 mL·min-1. The column temperature was 40℃. The analytes were detected by tandem mass spectrometry with the electrospray ionization(ESI-) source combined with multiple reaction monitoring(MRM) mode. RESULTS Psuedo-ginsenoside F11 showed good linear relationship within the ranges of 0.113 0-451.9 ng·mL-1(r=0.999), and the RSD of repeatability test was 3.5%. The average recovery(n=9) of pseudo-ginsenoside F11 was 96.9%. The limit of detection was 0.1 ng·mL-1 and the limit of quantitation was 0.4 ng·mL-1. CONCLUSION The established method is fast and accurate with a high sensitivity and a high degree of separation. It can be used for the screening and detection of Panax quinquefolium illegally added in Wuji Baifeng pills. |
| Key words: UPLC-MS/MS Wuji Baifeng pills Panax quinquefolium pseudo-ginsenoside F11 illegally added |
