| 引用本文: | 孔凡茹,田密,麻开香.响应面法优化缓解体力疲劳口服液的提取工艺[J].中国现代应用药学,2020,37(6):689-693. |
| KONG Fanru,TIAN Mi,MA Kaixiang.Optimization of Extraction Process for Relieving Physical Fatigue Oral Solution by Response Surface Methodology[J].Chin J Mod Appl Pharm(中国现代应用药学),2020,37(6):689-693. |
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| 摘要: |
| 目的 优化缓解体力疲劳口服液的提取工艺，为保健食品缓解体力疲劳口服液的制备和质量控制提供依据。方法 以黄芪甲苷、总皂苷、粗多糖含量为指标，在单因素试验的基础上，通过响应面法考察料液比、提取时间和提取次数对缓解体力疲劳口服液提取效果的影响，从而优化提取工艺。结果 通过响应面设计试验，综合考察3种有效成分的含量，得到最佳提取工艺为加水量12倍、提取2次，每次1.5 h。在此工艺条件下，提取黄芪甲苷、总皂苷、粗多糖含量分别为0.110 4，5.112 3，16.502 9 mg·mL-1，与预测值总体吻合。结论 通过响应面设计的提取工艺合理可行，含量测定结果可靠，为缓解体力疲劳口服液的提取制备和质量控制提供实验依据。 |
| 关键词: 缓解体力疲劳口服液 黄芪甲苷 总皂苷 粗多糖 提取工艺 响应面法 |
| DOI：10.13748/j.cnki.issn1007-7693.2020.06.010 |
| 分类号:R284.2 |
| 基金项目: |
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| Optimization of Extraction Process for Relieving Physical Fatigue Oral Solution by Response Surface Methodology |
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KONG Fanru, TIAN Mi, MA Kaixiang
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Beijing Zhendong Guangming Pharmaceutical Research Institute Co., LTD., Beijing 100089, China
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| Abstract: |
| OBJECTIVE To optimize the extraction process of relieving physical fatigue oral solution, and provide basis for the preparation and quality control of health food to relieving physical fatigue oral solution. METHODS The contents of astragaloside A, the total saponin and the crude polysaccharide were used as the index. Based on the single factor experiment, the effects of solid-liquid ratio, extraction time and extraction times on the extraction of physical fatigue oral solution were investigated by response surface methodology and in order to optimize extraction process. RESULTS Through the response surface design test, the contents of the three active ingredients were comprehensively investigated, and the best extraction process was obtained by adding 12 times of water and extracting 2 times for 1.5 h each time. Under the conditions of this process, the contents of astragaloside A, the total saponin and the crude polysaccharide were 0.110 4, 5.112 3, 16.052 9 mg·mL-1, respectively, which were consistent with the predicted values. CONCLUSION The extraction process designed by response surface is reasonable and feasible, and the results of determinated content are reliable. It provides experimental basis for the preparation and quality control of relieving physical fatigue oral solution. |
| Key words: relieving physical fatigue oral solution astragaloside A total saponin crude polysaccharide extraction process response surface method |
