| 引用本文: | 洪诚韬,陈君,陆美斐,孙洋,乔洪翔.HPLC同时测定黄芩花中野黄芩苷和黄芩苷的含量[J].中国现代应用药学,2020,37(11):1320-1323. |
| HONG Chengtao,CHEN Jun,LU Meifei,SUN Yang,QIAO Hongxiang.Simultaneous Determination of Scutellarin and Baicalin in the Flowers of Scutellaria Baicalensis Georgi by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2020,37(11):1320-1323. |
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| 摘要: |
| 目的 建立HPLC同时测定黄芩花中野黄芩苷和黄芩苷含量的方法，并控制其质量。方法 收集不同时期（初花期、盛花期、凋花期）黄芩花样品，采用HPLC测定其中的野黄芩苷和黄芩苷含量。色谱柱ZORBAX Eclipse XDB-C18（4.6 mm×250 mm，5 μm），流动相为甲醇-水-磷酸（47：53：0.2），流速1.0 mL·min-1，检测波长280 nm；柱温30℃。结果 黄芩花中，野黄芩苷平均含量为1.690%，黄芩苷平均含量为0.306%；8月中旬盛花期时，2种指标成分含量最高，分别为1.857%，0.360%。结论 本法测定黄芩花中野黄芩苷和黄芩苷的含量简便、准确、快捷，可用于黄芩花的质量控制。 |
| 关键词: 黄芩花 野黄芩苷 黄芩苷 高效液相色谱法 |
| DOI：10.13748/j.cnki.issn1007-7693.2020.11.007 |
| 分类号:R917.101 |
| 基金项目:浙江省教育厅项目（Y201635995） |
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| Simultaneous Determination of Scutellarin and Baicalin in the Flowers of Scutellaria Baicalensis Georgi by HPLC |
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HONG Chengtao1, CHEN Jun2, LU Meifei1, SUN Yang1, QIAO Hongxiang2
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1.Department of Pharmacy, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center of Child Health, Hangzhou 310003, China;2.Yunnan Institute for Botany of Conba Group, Kunming 650217, China
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| Abstract: |
| OBJECTIVE To establish a method for the determination of scutellarin and baicalin in the flowers of Scutellaria baicalensis Georgi by HPLC, to control its quality. METHODS The contents of scutellarin and baicalin in the flowers collected at different developmental stages(Chuhua stage, Shenghua stage and Diaohua stage) in Scutellaria baicalensis Georgi were measured by HPLC. The chromatographic column was ZORBAX Eclipse XDB-C18(4.6 mm×250 mm, 5 μm). The moblie phase was methanol-water-phosphoric acid(47:53:0.2), flow rate was 1.0 mL·min-1, detection wavelength was 280 nm, column temperature was 30℃. RESULTS The average contents of scutellarin and baicalin were 1.690% and 0.306%, respectively. The highest content of scutellarin and baicalin were 1.857% and 0.360%, respectively, which in the flowers collected at August. CONCLUSION The method is rapid convenient and accurate, and is suitable for the quality control of the flowers of Scutellaria baicalensis Georgi. |
| Key words: flowers of Scutellaria baicalensis Georgi scutellarin baicalin HPLC |
