| 引用本文: | 周颖,严伟,李晴宇,董蓉,马志媛,林能明.岩藻糖氟化模拟类似物对乳腺癌细胞增殖、侵袭的影响及机制研究[J].中国现代应用药学,2020,37(2):143-148. |
| ZHOU Ying,YAN Wei,LI Qingyu,DONG Rong,MA Zhiyuan,LIN Nengming.Effect and Mechanism of Fucose Fluoride Analogue Suppresses Breast Cancer Cell Proliferation and Migration[J].Chin J Mod Appl Pharm(中国现代应用药学),2020,37(2):143-148. |
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| 岩藻糖氟化模拟类似物对乳腺癌细胞增殖、侵袭的影响及机制研究 |
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周颖1,2, 严伟1, 李晴宇1, 董蓉1,2, 马志媛1,2, 林能明1,2
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1.浙江大学医学院附属杭州市第一人民医院药学部临床药学科, 杭州 310006;2.浙江大学医学院附属杭州市第一人民医院浙江省临床肿瘤药理与毒理学研究重点实验室, 杭州 310006
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| 摘要: |
| 目的 研究岩藻糖的氟化模拟类似物2-氟-L-岩藻糖（2-fluoro-L-fucose，2FF）对乳腺癌MDA-MB231细胞增殖和侵袭的影响，并探讨其可能的作用机制。方法 凝集素染色法检测2FF处理后乳腺癌细胞中核心岩藻糖的表达情况；CCK-8法测定2FF处理对乳腺癌细胞增殖能力的影响；Transwell实验法测定2FF处理后乳腺癌侵袭能力的变化；Westernblotting分析Hippo信号通路相关蛋白的表达情况。结果 2FF可显著抑制乳腺癌MDA-MB231细胞中核心岩藻糖的表达，呈剂量和时间依赖性；2FF处理后可以显著抑制乳腺癌MDA-MB231细胞的增殖和侵袭；Western blotting结果显示，2FF使用后，pYAP、pLATS、pMST1/2表达下降，总体YAP、LATS、MST1表达未见明显改变。结论 2FF可以抑制核心岩藻糖在乳腺癌细胞中的表达，并抑制乳腺癌细胞的增殖和迁移；其机制可能与Hippo信号通路的调节有关。 |
| 关键词: 岩藻糖氟化模拟类似物 乳腺癌 细胞增殖 细胞迁移 |
| DOI：10.13748/j.cnki.issn1007-7693.2020.02.004 |
| 分类号:R956.2 |
| 基金项目:国家自然科学基金项目（31900922）；浙江省医学重点学科（省市共建，浙卫办[2018]2号）；杭州市医学重点学科（杭卫计发[2017]51号） |
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| Effect and Mechanism of Fucose Fluoride Analogue Suppresses Breast Cancer Cell Proliferation and Migration |
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ZHOU Ying1,2, YAN Wei1, LI Qingyu1, DONG Rong1,2, MA Zhiyuan1,2, LIN Nengming1,2
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1.Affiliated Hangzhou First People's Hospital, Zhejiang University, School of Medicine, Department of Clinical Pharmacy, Hangzhou 310006, China;2.Affiliated Hangzhou First People's Hospital, Zhejiang University, School of Medicine, Key Laboratory of Clinical Cancer Pharmacology and Toxicology Research of Zhejiang Province, Hangzhou 310006, China
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| Abstract: |
| OBJECTIVE To investigate effect of fucose fluoride analogue(2-fluoro-L-fucose, 2FF) on the proliferation and invasion of breast cancer MDA-MB231 cells, and to explore the possible mechanism. METHODS Lectin blot was performed to detect the expression of core fucose in breast cancer cells after 2FF treatment. CCK-8 was used to determine the effect of 2FF treatment on the proliferation of breast cancer cells. The changes of invasive abilities of breast cancer cells after 2FF treatment were determined by Transwell assay and the expression of Hippo signaling pathway-related proteins were checked by Western blotting. RESULTS 2FF suppressed core fucosylation in the breast cancer, in a dose- and time-dependent manner. After treated by 2FF, the cell proliferation and migration was significantly inhibited. The expression of pYAP, pLATS, pMST1/2 was significantly decreased in the 2FF treated cells compared with the control cells. However, the expression of YAP, LATS, MST1 had little changes. CONCLUSION 2FF inhibit the expression of core fucosylation in the breast cancer cells, and inhibit the cell proliferation and migration. The mechanism may be related to the regulation of Hippo signaling pathway. |
| Key words: fucose fluoride analogue breast cancer cell proliferation cell migration |
