| 引用本文: | 阚微娜,杨宏伟.高效液相色谱法-荧光检测器测定复方氨基酸注射液中铝元素含量[J].中国现代应用药学,2020,37(21):2633-2636. |
| KAN Weina,YANG Hongwei.Determination of Aluminum in Compound Aminoacid Injection by HPLC-Fluorescence Detector[J].Chin J Mod Appl Pharm(中国现代应用药学),2020,37(21):2633-2636. |
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| 摘要: |
| 目的 建立复方氨基酸注射液中铝元素的高效液相色谱测定方法。方法 样品经酸水解后，游离态的铝元素与流动相中的8-羟基喹啉结合，形成荧光配合物，高效液相色谱-荧光检测器进行测定，采用标准曲线法计算铝元素的含量。结果 铝元素在5~200 μg·L-1线性关系良好，精密度、重复性的RSD均<3%，加样回收率在93.15%~110.07%。结论 方法易操作，灵敏度高，准确度好，为注射液中铝元素的含量测定提供有效的方法。 |
| 关键词: 复方氨基酸注射液 铝元素 高效液相色谱法 荧光检测器 8-羟基喹啉 |
| DOI：10.13748/j.cnki.issn1007-7693.2020.21.012 |
| 分类号:R917 |
| 基金项目: |
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| Determination of Aluminum in Compound Aminoacid Injection by HPLC-Fluorescence Detector |
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KAN Weina1,2, YANG Hongwei1,2
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1.Liaoning Inspection, Examination&2.Certification Centre, Liaoning Institute for Drug Control, Shenyang 110036, China
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| Abstract: |
| OBJECTIVE To establish a HPLC method for the determination of aluminum in compound aminoacid injections. METHODS After acid hydrolysis, the free aluminum was combined with 8-hydroxyquinoline in the mobile phase to form a fluorescent complex. HPLC and fluorescent detector was used to determine the content of aluminum by standard curve method. RESULTS The linear relationship of aluminum was good in the range of 5-200 μg·L-1. The precision and repeatability of RSD were <3%, and the recovery rates were 93.15%-110.07%. CONCLUSION The method is easy to operate, high sensitivity and good accuracy. It provides an effective method for the determination of aluminum in the injection. |
| Key words: compound aminoacid injection aluminum HPLC fluorescent detector 8-hydroxyquinoline |
