| 引用本文: | 黄芳,陈红斌,杜静.健胃合剂质量控制方法研究[J].中国现代应用药学,2019,36(11):1375-1378. |
| HUANG Fang,CHEN Hongbin,DU Jing.Study on Quality Control Method of Jianwei Mixture[J].Chin J Mod Appl Pharm(中国现代应用药学),2019,36(11):1375-1378. |
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| 摘要: |
| 目的 建立健胃合剂质量控制的方法。方法 采用薄层色谱法对甘草、黄连和麸炒枳壳进行定性鉴别；采用HPLC测定柚皮苷含量。色谱柱为Diamonsil C18（250 mm×4.6 mm，5 μm），流动相为甲醇-0.2%磷酸（40：60），检测波长为283 nm，流速为1.0 mL·min-1，柱温为30℃，进样量为5 μL。结果 建立的薄层色谱法样品斑点清晰，分离度好。含量测定结果显示，柚皮苷在0.005 33~0.085 23 mg·mL-1内线性关系良好，平均回收率为99.42%，RSD为0.76%。结论 该方法准确可靠，专属性好，可以用于健胃合剂的质量控制。 |
| 关键词: 健胃合剂 甘草 黄连 麸炒枳壳 柚皮苷 质量控制 |
| DOI：10.13748/j.cnki.issn1007-7693.2019.11.013 |
| 分类号:R284.1 |
| 基金项目: |
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| Study on Quality Control Method of Jianwei Mixture |
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HUANG Fang1, CHEN Hongbin2, DU Jing1
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1.Zhejiang Integrated Traditional and Western Medicine Hospital, Hangzhou 310003, China;2.Zhejiang Huisong Pharmaceutical Co., Ltd., Hangzhou 310005, China
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| Abstract: |
| OBJECTIVE To establish the quality control method of Jianwei mixture. METHODS Thin layer chromatography(TLC) was used to identify Glycyrrhizae Radix et Rhizoma, Coptidis Rhizoma, stir-baked Aurantii Fructus in bran. Determination of naringin content by HPLC. The mobile phase for determining naringin was methanol-0.2% phosphoric acid(40:60), the UV detection wavelength was 283 nm, Diamonsil C18(250 mm×4.6 mm, 5 μm) column was used for the determination, and the flow rate was 1.0 mL·min-1, the column temperature was 30℃ and the injection volume was 5 μL. RESULTS TLC showed clear spots and good resolution. The linearity of naringin was in the range of 0.005 33-0.085 23 mg·mL-1, the average recovery of naringin was 99.42%, RSD was 0.76%. CONCLUSION The method is accurate, reliable and exclusive, it can be used for the quality control of Jianwei mixture. |
| Key words: Jianwei mixture Glycyrrhizae Radix et Rhizoma Coptidis Rhizoma stir-baked Aurantii Fructus in bran naringin quality control |
