| 引用本文: | 董福越,徐科一,黄远.HPLC同时测定板蓝根颗粒中2种关键抗病毒成分的含量[J].中国现代应用药学,2019,36(18):2245-2248. |
| DONG Fuyue,XU Keyi,HUANG Yuan.Simultaneous Determination of two Antiviral Components in Banlangen Granules by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2019,36(18):2245-2248. |
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| 摘要: |
| 目的 建立HPLC同时测定板蓝根颗粒中（R，S）-告衣春和直铁线莲宁B含量的方法。方法 采用Agilent ZORBAX SB-C18色谱柱（250 mm×4.6 mm，5μm），流动相为甲醇-水，梯度洗脱，流速为0.8 mL·min-1，进样量为10μL，柱温为30℃，检测波长230 nm。结果 （R，S）-告衣春、直铁线莲宁B在2.39~47.80μg·mL-1（r=1.000 0）和3.18~63.60μg·mL-1（r=0.999 9）内线性关系良好，平均加样回收率分别为100.90%（RSD=1.56%）和99.48%（RSD=1.08%）。结论 本方法操作简便、准确稳定、专属性强、重复性好，可为提高板蓝根颗粒质量标准提供依据。 |
| 关键词: 板蓝根颗粒 (R,S)-告衣春 直铁线莲宁B 高效液相色谱法 |
| DOI：10.13748/j.cnki.issn1007-7693.2019.18.002 |
| 分类号:R284.1 |
| 基金项目:中国博士后科学基金面上资助项目（2017M622652） |
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| Simultaneous Determination of two Antiviral Components in Banlangen Granules by HPLC |
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DONG Fuyue, XU Keyi, HUANG Yuan
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Hutchison Whampoa Guangzhou Baiyunshan Chinese Medicine Company Limited, Guangzhou 510515, China
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| Abstract: |
| OBJECTIVE To establish a HPLC method for the simultaneous content determination of (R,S)-goitrin and clemastanin B in banlangen granules. METHODS The analysis methanol was performed on a Agilent ZORBAX SB-C18 column(250 mm×4.6 mm, 5 µm), the mobile phase was methanol-water flowing of gradient elution, the flow rate was 0.8 mL·min-1, the injection volume was 10 µL, the column temperature was 30℃, and the detection wavelength was 230 nm. RESULTS (R,S)-goitrin and clemastanin B showed good linear relationships within their own ranges of 2.39-47.80 µg·mL-1 (r=1.000 0) and 3.18-63.60 µg·mL-1(r=0.999 9). The average recoveries were 100.90%(RSD=1.56%) and 99.48%(RSD=1.08%). CONCLUSION This method is simple, accurate, specific and reproducible, can provide a basis for raising banlangen granules quality standards. |
| Key words: Banlangen granules (R,S)-goitrin clemastanin B HPLC |
