| 引用本文: | 程世云,张亚中,韩玲玲,王浩,王满媛,周志凌.HPLC同时测定肺宁制剂中4种成分的含量[J].中国现代应用药学,2019,36(22):2814-2817. |
| CHENG Shiyun,ZHANG Yazhong,HAN Lingling,WANG Hao,WANG Manyuan,ZHOU Zhiling.Simultaneous Determination of Four Constituents in Feining Preparation by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2019,36(22):2814-2817. |
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| 摘要: |
| 目的 建立HPLC同时测定肺宁颗粒、肺宁胶囊和肺宁片中新绿原酸、绿原酸、隐绿原酸和金丝桃苷4种成分的含量。方法 采用Waters XBridgeTMC18色谱柱（4.6 mm×250 mm，5 μm），流动相为乙腈-0.4%磷酸水溶液，梯度洗脱，流速1.0 mL·min-1；检测波长327，353 nm；柱温30℃。结果 4种成分在各自范围内线性关系良好（r>0.9990），平均加样回收率96.1%~104.7%，RSD为0.4%~1.6%。结论 该方法准确、可靠，重复性好，可用于肺宁制剂的质量控制。 |
| 关键词: 肺宁颗粒 肺宁胶囊 肺宁片 新绿原酸 绿原酸 隐绿原酸 金丝桃苷 高效液相色谱法 |
| DOI：10.13748/j.cnki.issn1007-7693.2019.22.013 |
| 分类号:R917.101 |
| 基金项目:2018年国家药品抽验计划项目（食药监药化监[2017]131号） |
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| Simultaneous Determination of Four Constituents in Feining Preparation by HPLC |
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CHENG Shiyun, ZHANG Yazhong, HAN Lingling, WANG Hao, WANG Manyuan, ZHOU Zhiling
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Anhui Provincial Institute for Food and Drug Control, Hefei 230051, China
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| Abstract: |
| OBJECTIVE To establish an HPLC method for the simultaneous determination of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid and hyperin in Feining granules, Feining capsules and Feining tablets. METHODS Preparation was performed on a 30℃ Waters XBridgeTM C18 column(4.6 mm×250 mm, 5 μm), with the mobile phase comprising of acetonitrile-0.4% phosphoric acid flowing at 1.0 mL·min-1 in a gradient elution manner, and the detection wavelengths were set at 327, 353 nm. RESULTS Four constituents showed good linear relationships with their own ranges(r>0.999 0), while the average recoveries were 96.1%-104.7% with the RSDs of 0.4%-1.6%. CONCLUSION This accurate, reliable and reproducible method can be used for the quality control of Feining preparation. |
| Key words: Feining granules Feining capsules Feining tablets neochlorogenic acid chlorogenic acid cryptochlorogenic acid hyperin HPLC |
