| 引用本文: | 傅萍,刘峰,谢华.离子色谱法测定小儿复方氨基酸注射液中的氨含量[J].中国现代应用药学,2019,36(18):2295-2298. |
| FU Ping,LIU Feng,XIE Hua.Determination of ammonium in pediatric compound aminoacid injection by Ion Chromatograpy[J].Chin J Mod Appl Pharm(中国现代应用药学),2019,36(18):2295-2298. |
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| 摘要: |
| 目的 采用离子色谱法优化测定小儿复方氨基酸注射液中的氨含量。方法 采用阳离子交换色谱柱RFICTM IonPac® CG16（4 mm×50 mm），保护柱CS16（4 mm×250 mm）；以20 mmol·L-1甲基磺酸为流动相A，以100 mmol·L-1甲基磺酸为流动相B，梯度洗脱，流速为1.0 mL·min-1；检测器为电导检测器（带抑制器CERS 300，4 mm）；进样体积25 μl。结果 氨的线性范围为0.149 9~5.997 9 μg·ml-1（r=0.998 4），平均回收率为96.8%，RSD为2.82%。结论 该法专属性高、结果准确可靠，可用于小儿复方氨基酸注射液中氨的含量控制。 |
| 关键词: 离子色谱法 阳离子交换柱 电导检测器 小儿复方氨基酸注射液 氨 含量测定 |
| DOI：10.13748/j.cnki.issn1007-7693.2019.18.012 |
| 分类号:R917.101 |
| 基金项目: |
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| Determination of ammonium in pediatric compound aminoacid injection by Ion Chromatograpy |
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FU Ping, LIU Feng, XIE Hua
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Sichuan food and drug inspection and testing institute, Chengdu 611731, China
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| Abstract: |
| OBJECTIVE To use ion chromatography method to optimize the assay of ammonium in pediatric compound aminoacid injection. METHODS The IC method was performed on a cation exchange column RFICTM IonPac® CG16 (4 mm×50 mm), CS16(4 mm×250 mm) by gradient elution at a flow rate of 1.0 mL·min-1. The mobile phase A was 20 mmol·L-1 methanesulfonic acid, the mobile phase B was 100 mmol·L-1 methanesulfonic acid. The detector was conductivity detector with a suppressor(CERS 300, 4 mm) and the injection volume was 25 μl. RESULTS The linear range of the ammonium was 0.149 9-5.997 9 μg·mL-1(r=0.998 4). The average recovery of it was 96.8% and the RSD was 2.82%. CONCLUSION The method was specific, accurate and suitable for the assay of ammonium for quantitative control of pediatric compound aminoacid injection. |
| Key words: ion chromatography cation exchange column conductivity detector pediatric compound aminoacid injection ammonium content determination |
