| 引用本文: | 唐登峰,马临科,龚青,方翠芬.CO2超临界流体色谱法同时测定莪术油中3个倍半萜类成分的含量[J].中国现代应用药学,2019,36(20):2539-2542. |
| TANG Dengfeng,MA Linke,GONG Qing,FANG Cuifen.Simultaneous Determination of Three Sesquiterpenoids in the Zedoary Turmeric Oil by CO2-Supercritical Fluid Chromatography[J].Chin J Mod Appl Pharm(中国现代应用药学),2019,36(20):2539-2542. |
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| 摘要: |
| 目的 建立CO2超临界流体色谱法测定莪术油中呋喃二烯、牻牛儿酮和莪术二酮含量的方法。方法 采用ACQUITY UPC2 HSS C18 SB色谱柱（3.0 mm×150 mm，1.8 μm），以CO2-乙腈为流动相，梯度洗脱；流速为1.0 mL·min-1；检测波长为216 nm，柱温为55℃，背压为2 000 psi。结果 呋喃二烯在2.67~1 337.26μg·mL-1内线性关系良好（r=1.000），加样回收率为97.94%（n=6，RSD=1.50%）。牻牛儿酮在2.77~1 386.00 μg·mL-1内线性关系良好（r=1.000），加样回收率为96.07%（n=6，RSD=1.68%）；莪术二酮在6.99~3 493.00 μg·mL-1内线性关系良好（r=1.000），加样回收率为99.33%（n=6，RSD=1.88%）。结论 本方法快捷准确、稳定且绿色环保，可用于莪术油中上述3个倍半萜类成分的质量控制。 |
| 关键词: CO2超临界流体色谱法 呋喃二烯 牻牛儿酮 莪术二酮 莪术油 |
| DOI：10.13748/j.cnki.issn1007-7693.2019.20.010 |
| 分类号:R917.101 |
| 基金项目:浙江省科技计划项目（2016C37064） |
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| Simultaneous Determination of Three Sesquiterpenoids in the Zedoary Turmeric Oil by CO2-Supercritical Fluid Chromatography |
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TANG Dengfeng, MA Linke, GONG Qing, FANG Cuifen
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Zhejiang Institute for Food and Drug Control, Hangzhou 310052, China
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| Abstract: |
| OBJECTIVE A CO2-Supercritical Fluid Chromatography(CO2-SFC) method was established for the simultaneous determination of furanodiene, curdione and germacrone in the Zedoary Turmeric Oil. METHODS A UPC2 HSS C18 SB analytical column(3.0 mm×150 mm, 1.8 μm) was adopted and the mobile phase consisted of CO2-acetonitrile with linear gradient elution(0-8 min, 99.5%→84% of CO2) at the flow rate of 1 mL·min-1. The detection wavelength was 216 nm, the column temperature was maintained at 55℃, and the back pressure was was kept at 2 000 psi. RESULTS The linear range of furanodiene was 2.67-1 337.26 μg·mL-1 (r=1.000), and the average recovery(n=6) was 97.94% with RSD of 1.50%. The linear range of curdione was 2.77-1 386.00 μg·mL-1 (r=1.000), and the average recovery(n=6) was 96.07% with RSD of 1.68%. The linear range of germacrone was 6.99-3 493.00 μg·mL-1 (r=1.000), and the average recovery(n=6) was 99.33% with RSD of 1.88%. CONCLUSION The established method is rapid, accurate, and it can protect environment, which provides a new method for the quality control of furanodiene, curdione and germacrone in the Zedoary Turmeric Oil. |
| Key words: CO2-supercritical fluid chromatography furanodiene curdione germacrone Zedoary Turmeric oil |
