| 引用本文: | 施琦渊,吴家琪,於佳露,张心怡,李钦,褚建波,施菁.3,5-O-二咖啡酰基奎宁酸的制备及其对HeLa细胞的抗增殖活性研究[J].中国现代应用药学,2019,36(14):1738-1742. |
| SHI Qiyuan,WU Jiaqi,YU Jialu,ZHANG Xinyi,LI Qin,CHU Jianbo,SHI Jing.Preparation of 3,5-O-dicaffeoyl Quinic Qcid and Its Antiproliferative Activity on HeLa Cells[J].Chin J Mod Appl Pharm(中国现代应用药学),2019,36(14):1738-1742. |
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| 3,5-O-二咖啡酰基奎宁酸的制备及其对HeLa细胞的抗增殖活性研究 |
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施琦渊1, 吴家琪1, 於佳露1, 张心怡1, 李钦2, 褚建波1, 施菁1
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1.杭州医学院, 杭州 310053;2.浙江省医学科学院药物研究所, 杭州 310013
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| 摘要: |
| 目的 制备高纯度3，5-O-二咖啡酰基奎宁酸，并评价其对人宫颈癌HeLa细胞的抗增殖活性。方法 采用柱色谱提取法和中压液相色谱法从奇蒿花中分离、纯化得到高纯度的3，5-O-二咖啡酰基奎宁酸。采用MTT法评价该化合物对人宫颈癌HeLa细胞的体外抗增殖活性。结果 柱色谱提取的提取率和中压液相色谱法的回收率分别为99.0%，61.2%，总回收率为54.0%。随着3，5-O-二咖啡酰基奎宁酸浓度升高，HeLa细胞存活率下降，细胞形态损伤增加，受试药物的IC50值为26.5µg·mL-1。结论 本研究提供了一种简单、高效、节能的3，5-O-二咖啡酰基奎宁酸制备方法。3，5-O-二咖啡酰基奎宁酸对HeLa细胞具有一定的体外抗增殖活性。 |
| 关键词: 3,5-O-二咖啡酰基奎宁酸 奇蒿 柱色谱提取法 中压液相色谱法 抗增殖活性 |
| DOI：10.13748/j.cnki.issn1007-7693.2019.14.003 |
| 分类号:R285.5 |
| 基金项目:浙江省医药卫生项目（2017KY311）；"十三五"浙江省中医药（中西医结合）重点学科建设计划项目（2017-XK-A43） |
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| Preparation of 3,5-O-dicaffeoyl Quinic Qcid and Its Antiproliferative Activity on HeLa Cells |
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SHI Qiyuan1, WU Jiaqi1, YU Jialu1, ZHANG Xinyi1, LI Qin2, CHU Jianbo1, SHI Jing1
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1.Hangzhou Medical College, Hangzhou 310053, China;2.Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China
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| Abstract: |
| Objective To study the preparing technology of 3,5-O-dicaffeoyl quinic acid with high purity and its antiproliferative activity on HeLa cells. Methods 3,5-O-dicaffeoyl quinic acid was prepared by column chromatographic extraction methods coupled with medium pressure liquid chromatography from flower of Artemisia anomala. The antiproliferative activity of 3,5-O-dicaffeoyl quinic acid on HeLa cell was evaluated by MTT method. Results The extraction and purification recovery yield were 99.0% and 61.2%, respectively. The total recovery yield of 3,5-O-dicaffeoyl quinic acid reached 54.0%. With the increase of 3,5-O-dicaffeoyl quinic acid concentration, the HeLa cells viability decreased and the cell morphological damage increased. The IC50 value of 3,5-O-dicaffeoyl quinic acid on HeLa cells was 26.5 µg·mL-1. Conclusion A simple, efficient, energy-saving 3,5-O-dicaffeoyl quinic acid preparation method is developed. The compound displays good antiproliferative activity on HeLa cells in vitro. |
| Key words: 3,5-O-dicaffeoyl quinic acid Artemisia anomala column chromatographic extraction method medium pressure liquid chromatography antiproliferative activity |
