| 引用本文: | 邱军强,苗晶囡,张华,刘树民.黑木耳多糖顺铂配合物抗HeLa和LoVo细胞增殖活性[J].中国现代应用药学,2019,36(14):1729-1732. |
| QIU Junqiang,MIAO Jingnan,ZHANG Hua,LIU Shumin.Antiproliferative Activity of Auricularia Auricula Polysaccharide-cis-diaminedichloroplatinum Complex Against HeLa and LoVo Cell[J].Chin J Mod Appl Pharm(中国现代应用药学),2019,36(14):1729-1732. |
|
| 摘要: |
| 目的 研究不同分子量黑木耳多糖（Auricularia auricula polysaccharide，AAP）能否与顺铂形成配合物，同时研究其配合物对HeLa和LoVo细胞的抗肿瘤细胞增殖活性。方法 采用"One pot"法合成不同分子量的黑木耳多糖顺铂配合物（Auricularia auricula polysaccharide-cis-diaminedichloroplatinum，AAP-CDDP），并应用MTT法对其体外抗肿瘤细胞增殖活性进行研究。结果 AAP-CDDP的抑制HeLa和LoVo肿瘤细胞增殖活性与其螯合顺铂量密切相关，相关系数r2分别为0.877 5和0.913 2；其中AAP（Ⅴ）-CDDP具有最强的抗肿瘤细胞增殖活性。结论 AAP与顺铂形成配合物具有很好的抗肿瘤细胞增殖活性。 |
| 关键词: 黑木耳多糖 顺铂 配合物 抗肿瘤 |
| DOI：10.13748/j.cnki.issn1007-7693.2019.14.001 |
| 分类号:R285.5 |
| 基金项目:国家自然科学青年基金项目（31401483）；黑龙江省博士后基金（LBH-Z14098）；黑龙江省科学基金项目（C2018034） |
|
| Antiproliferative Activity of Auricularia Auricula Polysaccharide-cis-diaminedichloroplatinum Complex Against HeLa and LoVo Cell |
|
QIU Junqiang1, MIAO Jingnan1, ZHANG Hua2, LIU Shumin3
|
|
1.School of Pharmacy, Hainan Medical University, Haikou 570100, China;2.School of Chemistry Engineering, Harbin Institute of Technology, Harbin 150001, China;3.School of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150090, China
|
| Abstract: |
| OBJECTIVE To study whether Auricularia auricula polysaccharide(AAP) can form complexes with cisplatin, and study the anti-tumor cell proliferation activity of the complexes against HeLa and LoVo cells. METHODS Auricularia auricula polysaccharide-cis-diaminedichloroplatinum(AAP-CDDP) complexes with different molecular weights were synthesized using "One pot" method, and their anti-tumor cell proliferation activity in vitro were studied by MTT method. RESULTS The inhibition activity of AAP-CDDP on the proliferation of HeLa and LoVo cervical cancer cells was closely related to the amount of cisplatin in AAP-CDDP, and the correlation coefficients of r2 were 0.877 5 and 0.913 2, respectively. Among them, AAP(V)-CDDP had the strongest anti-tumor cell proliferation activity. CONCLUSION The complex of AAP and cisplatin has good anti-tumor cell proliferation activity. |
| Key words: Auricularia auricula polysaccharide CDDP complex anti-tumor |
