| 引用本文: | 申坤容,魏含笑,李永福,王英骥.PPARγ激动剂荧光探针法研究进展[J].中国现代应用药学,2020,37(1):120-124. |
| SHEN Kunrong,WEI Hanxiao,LI Yongfu,WANG Yingji.Progress in Fluorescent Probes for PPAR Gamma Agonists[J].Chin J Mod Appl Pharm(中国现代应用药学),2020,37(1):120-124. |
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| 摘要: |
| 过氧化物酶增殖激活受体（peroxisome proliferator activated receptors，PPARs）属于核激素受体家族中的配体激活受体，包括3种亚型：PPARα、PPARβ/δ和PPARγ。PPARγ具有增强机体对胰岛素敏感性，调节体内糖平衡以及脂肪分化、生成等多种生物学功能。通过荧光探针法研究PPARγ与配体结合，对研究PPARγ激动剂作用机制及筛选PPARγ激动剂具有重要的意义。本文针对新型荧光探针法探究PPARγ与配体的结合能力以及筛选PPARγ激动剂研究进行综述。 |
| 关键词: PPAR 激动剂 荧光探针法 亲和力 |
| DOI：10.13748/j.cnki.issn1007-7693.2020.01.024 |
| 分类号:R917 |
| 基金项目:国家大学生创新创业训练计划项目（201710226019，201810226076） |
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| Progress in Fluorescent Probes for PPAR Gamma Agonists |
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SHEN Kunrong, WEI Hanxiao, LI Yongfu, WANG Yingji
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College of Pharmacy, Harbin Medical University, Harbin 150081, China
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| Abstract: |
| Peroxisome proliferator activated receptors(PPARs) are ligand-activated receptors in the nuclear hormone receptor family, including three subtypes:PPARα, PPARβ/δ and PPARγ. PPARγ has many biological functions, such as enhancing insulin sensitivity, regulating sugar balance, adipose differentiation and formation. The study of PPARγ binding to ligand by fluorescent probe method has great significance for studying the mechanism of PPARγ agonist and screening PPARγ agonists. This review summarizes the novel fluorescent probe method to explore the binding ability of PPARγ to ligands and the screening of PPARγ agonists. |
| Key words: PPAR agonist fluorescence probe affinity |
