| 引用本文: | 陈梦娇,王华,陈锦超,赵安,胡海红,曾苏.地西他滨诱导miR-200c/141表达抑制肾癌细胞侵袭迁移[J].中国现代应用药学,2019,36(13):1601-1607. |
| CHEN Mengjiao,WANG Hua,CHEN Jinchao,ZHAO An,HU Haihong,ZENG Su.Decitabine Inhibit Invasion and Migration Ability of Renal Cell Carcinoma Cells by Up-regulating MiR-200c/141[J].Chin J Mod Appl Pharm(中国现代应用药学),2019,36(13):1601-1607. |
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| 地西他滨诱导miR-200c/141表达抑制肾癌细胞侵袭迁移 |
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陈梦娇1, 王华2, 陈锦超2, 赵安2, 胡海红1, 曾苏1
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1.浙江大学药学院, 浙江省抗肿瘤药物临床前研究重点实验室, 杭州 310058;2.浙江省肿瘤医院, 杭州 310022
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| 摘要: |
| 目的 探讨DNA甲基转移酶抑制剂地西他滨对人肾细胞癌786-O和769-P细胞侵袭迁移能力的影响，并进行相关机制的研究。方法 实时荧光定量PCR法检测肾癌及其癌旁组织中miR-200c/141的表达量及2.5 μmol·L-1地西他滨对肾癌细胞系miR-200c/141的诱导作用；Transwell试验、划痕试验分别检测地西他滨、miR-200c/141对细胞侵袭迁移的影响；实时荧光定量PCR法、蛋白印迹法检测786-O和769-P细胞系钙黏蛋白表达量。结果 与正常肾癌旁组织相比，miR-200c/141在肾癌组织中的表达量显著下调。2.5 μmol·L-1地西他滨能诱导786-O和769-P细胞系中miR-200c/141的表达增加。地西他滨、miR-200c/141均能抑制细胞的侵袭迁移能力。786-O和769-P细胞经地西他滨处理后，与上皮间充质转化相关的钙黏蛋白表达水平显著上调。结论 地西他滨可以通过上调miR-200c/141的表达水平减弱肾癌细胞系的侵袭迁移能力。 |
| 关键词: 地西他滨 miR-200c miR-141 肾细胞癌 侵袭迁移 |
| DOI：10.13748/j.cnki.issn1007-7693.2019.13.001 |
| 分类号:R965.1 |
| 基金项目:国家重点研发计划（2017YFC0908600）；国家自然科学基金项目（81773817） |
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| Decitabine Inhibit Invasion and Migration Ability of Renal Cell Carcinoma Cells by Up-regulating MiR-200c/141 |
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CHEN Mengjiao1, WANG Hua2, CHEN Jinchao2, ZHAO An2, HU Haihong1, ZENG Su1
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1.College of Pharmaceutical Sciences, Zhejiang Province Key Laboratory of Anti-Cancer Drug Research, Zhejiang University, Hangzhou 310058, China;2.Cancer Hospital of Zhejiang Province, Hangzhou 310022, China
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| Abstract: |
| OBJECTIVE To investigate the effect and mechanism of DNA methyltransferase inhibitor decitabine on the invasion and migration ability of renal cancer cells 786-O and 769-P. METHODS The miR-200c/141 expression levels in renal cell carcinoma and its adjacent tissues were detected by RT-qPCR. MiR-200c/141 were induced by 2.5 μmol·L-1 decitabine and detected by RT-qPCR. Transwell assay and scratch assay were used to detect the effect of decitabine, miR-200c/141 on cell invasion and migration respectively. The mRNA and protein levels of E-cadherin were detected by RT-qPCR and western blot. RESULTS Compared with adjacent tissues, miR-200c/141 were significantly down regulated in renal cell carcinoma. The expression of miR-200c/141 could be induced in the 786-O and 769-P cells after treated with 2.5 μmol·L-1 decitabine. Scratch assay and Transwell assay results showed that decitabine, miR-200c/141 could inhibit cell migration and invasion. The expression of E-cadherin was upregulated in 786-O and 769-P cells after treated with decitabine, which associated with epithelial mesenchymal transition. CONCLUSION Decitabine can weaken the migration and invasion ability of 786-O and 769-P cells by up-regulating the expression of miR-200c/141. |
| Key words: decitabine miR-200c miR-141 renal cell carcinoma invasion and migration |
