| 引用本文: | 龙华晴,赵文慧,聂晓静,马津真,吴悦,陈璇,陈宇,吴人照.西洋参醇提物对慢性萎缩性胃炎大鼠的逆转效应研究[J].中国现代应用药学,2019,36(17):2131-2135. |
| LONG Huaqing,ZHAO Wenhui,NIE Xiaojing,MA Jinzhen,WU Yue,CHEN Xuan,CHEN Yu,WU Renzhao.Reversal Effect of American Ginseng Alcohol Extracts on Rats with Chronic Atrophic Gastritis[J].Chin J Mod Appl Pharm(中国现代应用药学),2019,36(17):2131-2135. |
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| 西洋参醇提物对慢性萎缩性胃炎大鼠的逆转效应研究 |
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龙华晴1,2, 赵文慧1,2, 聂晓静1,2, 马津真1,2, 吴悦1,2, 陈璇1, 陈宇1, 吴人照1
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1.浙江省中医药研究院, 杭州 310007;2.浙江中医药大学, 杭州 310053
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| 摘要: |
| 目的 探讨西洋参醇提物对亚硝基胍（N-methyl-N'-nitro-N-nitrosoguanidine，MNNG）诱发慢性萎缩性胃炎（chronic atrophic gastritis，CAG）模型大鼠的萎缩逆转作用及对PCNA蛋白、Bcl-2蛋白、PCNA mRNA、Bcl-2 mRNA、TFF3 mRNA、TNF-α mRNA的影响。方法 Wistar大鼠自由饮用浓度为167 μg·mL-1的MNNG饮用液，实验造模12周。确定成模后，将大鼠分为模型组、正常组、西洋参醇提物组。并分别给予药物干预后，观察大鼠体质量，胃组织病理组织学，血清胃泌素（gastrin，GAS），胃组织PCNA蛋白、Bcl-2蛋白、PCNA mRNA、Bcl-2 mRNA、TFF3 mRNA、TNF-α mRNA表达情况。结果 治疗第8周西洋参醇提物组体质量恢复显著高于模型组（P<0.05）。西洋参醇提物组腺体萎缩、不典型增生、肠化显著减轻（P<0.01）。西洋参醇提物组炎症等级、GAS较模型组无显著性差异。西洋参醇提物组PCNA蛋白、Bcl-2蛋白较模型组显著降低（P<0.01）。西洋参醇提物组PCNA mRNA、TFF3 mRNA、TNF-α mRNA较模型组均有下降，但差异无统计学意义；Bcl-2 mRNA较模型组有显著升高（P<0.01）。结论 西洋参醇提物对CAG模型大鼠具有良好的治疗作用，减轻体质量丢失，减轻胃黏膜萎缩和肠化生，其作用机制之一可能是通过下调胃黏膜PCNA蛋白、Bcl-2蛋白的高表达，起到改善乃至逆转CAG病理状态的作用。 |
| 关键词: 西洋参 醇提物 慢性萎缩性胃炎 增殖细胞核抗原 Bcl-2 三叶因子 肿瘤坏死因子-α 蛋白 mRNA |
| DOI：10.13748/j.cnki.issn1007-7693.2019.17.004 |
| 分类号:R285.5 |
| 基金项目:重大新药创制国家科技重大专项（2016ZX09101069）；国家自然科学基金项目（81703789）；浙江省自然科学基金（LY15H280011）；浙江省重大科技专项（2009C13032） |
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| Reversal Effect of American Ginseng Alcohol Extracts on Rats with Chronic Atrophic Gastritis |
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LONG Huaqing1,2, ZHAO Wenhui1,2, NIE Xiaojing1,2, MA Jinzhen1,2, WU Yue1,2, CHEN Xuan1, CHEN Yu1, WU Renzhao1
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1.Research Institute of Chinese Medicine in Zhejiang Province, Hangzhou 310007, China;2.Zhejiang University of Traditional Chinese Medicine, Hangzhou 310053, China
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| Abstract: |
| OBJECTIVE To discuss the reverse effect of gastric atrophy and the influences to PCNA and Bcl-2 protein, PCNA mRNA, Bcl-2 mRNA, TFF3 mRNA, TNF-α mRNA of American Ginseng alcohol extracts for the treatment of the model rats with chronic atrophic gastritis(CAG) induced by N-methyl-N'-nitro-N-nitrosoguanidine(MNNG). METHODS Wistar rats were free to drink MNNG solution with a concentration of 167 μg·mL-1, and they were made models for 12 weeks. After making the model successfully, divided the rats into model group, normal group and American Ginseng alcohol extracts group. After giving drugs intervention separately, observed the weight of rats, gastric tissue histopathology, gastrin(GAS), the expression of PCNA and Bcl-2 protein and mRNA, TFF3, TNF-α mRNA in gastric tissue. RESULTS In the eighth therapeutic week, the weight of rats in the American Ginseng alcohol extracts group were significantly higher than the model group(P<0.05). The glandular atrophy, atypical hyperplasia and intestinal metaplasia were significantly relieved in the American Ginseng alcohol extracts group(P<0.01). There was no significant difference of gastric mucosa inflammation level GAS between the American Ginseng alcohol extracts group and the model group. There were significant reduce of content of the PCNA protein and Bcl-2 protein in the American Ginseng alcohol extracts group(P<0.01). In the American Ginseng alcohol extracts group, there was a decline of PCNA mRNA, TFF3 mRNA and TNF-α mRNA but had not significant, Bcl-2 mRNA was significantly increased compared with model group(P<0.01). CONCLUSION American Ginseng alcohol extracts have a good therapeutic effect on the CAG model rats in reducing weight loss and reducing gastric atrophy and intestinal metaplasia, one of the mechanism may be down regulating the high expression of PCNA and Bcl-2 in gastric mucosa, and to improve and even reverse the pathological state of CAG. |
| Key words: American Ginseng alcohol extracts chronic atrophic gastritis proliferating cell nuclear antigen Bcl-2 trefoil factor 3 tumor necrosis factor-α(TNF-α) protein mRNA |
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