| 引用本文: | 张雅秋,乔俊缠,孙晓琦,王来兵,渠弼.和辉胶囊的质量标准研究[J].中国现代应用药学,2019,36(10):1240-1243. |
| ZHANG Yaqiu,QIAO Junchan,SUN Xiaoqi,WANG Laibing,QU Bi.Study on Quality Standard for Hehui Capsule[J].Chin J Mod Appl Pharm(中国现代应用药学),2019,36(10):1240-1243. |
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| 摘要: |
| 目的 建立和完善和辉胶囊的质量标准。方法 利用盐酸-镁粉反应鉴定沙棘中的总黄酮；采用薄层色谱法对丹参中的丹参酮ⅡA、荜茇中的胡椒碱以及制大黄中的大黄素分别进行鉴别；使用HPLC测定制剂中丹酚酸B的含量。结果 盐酸-镁粉反应呈阳性；薄层色谱斑点清晰，阴性对照无干扰；丹酚酸B在0.2029~1.622 9 μg内呈现良好的线性关系，r=0.999 8，平均加样回收率为98.83%，RSD为1.73%。结论 建立和完善的质量标准具有简便、可行、重复性好的特点，可用于该制剂的质量控制。 |
| 关键词: 和辉胶囊 质量标准 理化鉴别 薄层色谱 高效液相色谱 |
| DOI：10.13748/j.cnki.issn1007-7693.2019.10.014 |
| 分类号:R944.5 |
| 基金项目: |
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| Study on Quality Standard for Hehui Capsule |
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ZHANG Yaqiu, QIAO Junchan, SUN Xiaoqi, WANG Laibing, QU Bi
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School of Pharmacy, Inner Mongolia Medical University, Hohhot 010110, China
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| Abstract: |
| OBJECTIVE To establish and improve the quality standard for Hehui capsule. METHODS The total flavonoids in Hippophae rhamnoides were identified by hydrochloric acid-Mg reaction. The tanshinoneⅡA in Salvia miltiorrhiza Bge, the piperine in Piper longum, and the emodin in Rhubarb were identified by TLC, and the content of salvianolic acid B in the preparation was determined by HPLC. RESULTS The hydrochloric acid-Mg reaction was positive; the TLC spots were clear and the negative control had no interference. The salvianolic acid B showed a good linear relationship in the linear range of 0.202 9~1.622 9 μg, r=0.999 8, the average recovery rate was 98.83%, and RSD was 1.73%. CONCLUSION The established and improved quality standard is simple, feasible and reproducible, and can be used for the quality control of the preparation. |
| Key words: Hehui capsule quality standard physical and chemical identification TLC HPLC |
