| 引用本文: | 王治芬,张妮,马小攀,杨津,李,潘卫东,林昌虎.蜡梅不同部位精油化学成分分析及其体外抑菌活性研究[J].中国现代应用药学,2019,36(8):901-905. |
| WANG Zhifen,ZHANG Ni,MA Xiaopan,YANG Jin,LI Yan,PAN Weidong,LIN Changhu.Chemical Composition and in Vitro Antibacterial and Antifungal Activity of Essential Oils from Different Parts of Chimonanthus praecox (Linn.) Link[J].Chin J Mod Appl Pharm(中国现代应用药学),2019,36(8):901-905. |
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| 蜡梅不同部位精油化学成分分析及其体外抑菌活性研究 |
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王治芬1,2, 张妮2, 马小攀3, 杨津2, 李2, 潘卫东2, 林昌虎1,2
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1.贵州医科大学药学院, 贵阳 550004;2.贵州省中国科学院天然产物化学重点实验室, 贵阳 550014;3.德江县人民医院, 贵州 德江 565200
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| 摘要: |
| 目的 研究蜡梅Chimonanthus praecox（Linn.）Link根、叶、果皮中精油的化学组成及其抑菌活性。方法 采用气相色谱-质谱联用（GC-MS）技术对水蒸气蒸馏法提取的蜡梅根、叶、果皮精油进行成分分析，并分别采用肉汤二倍稀释法和菌丝生长法测试蜡梅不同部位精油对细菌和病原真菌的抑制活性。结果 鉴定了蜡梅根精油中的37种成分、蜡梅叶精油中的48种成分、蜡梅果皮精油中的38种成分，蜡酶不同部位精油中具有相同的化合物15种，分别占各部位精油总量的84.05%，36.83%，64.46%。抑菌实验结果表明蜡梅不同部位精油对受试菌均有不同强度的抑制作用。结论 蜡梅根精油对革兰氏阳性菌和病原真菌具有较强的抑菌活性，抑菌谱较广，且该精油含有多种生物活性成分，具有较高的药用价值及开发前景。 |
| 关键词: 蜡梅 精油 气相色谱-质谱联用 抑菌活性 |
| DOI：10.13748/j.cnki.issn1007-7693.2019.08.001 |
| 分类号:R284.1 |
| 基金项目:国家自然科学基金项目（81660580） |
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| Chemical Composition and in Vitro Antibacterial and Antifungal Activity of Essential Oils from Different Parts of Chimonanthus praecox (Linn.) Link |
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WANG Zhifen1,2, ZHANG Ni2, MA Xiaopan3, YANG Jin2, LI Yan2, PAN Weidong2, LIN Changhu1,2
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1.School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang 550004, China;2.Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550014, China;3.People's Hospital of Dejiang, Dejiang 565200, China
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| Abstract: |
| OBJECTIVE To study the chemical composition and antibacterial and antifungal activity of essential oil in root, leaf and peel of Chimonanthus praecox (Linn.) Link. METHODS The essential oils from the three parts were extracted by steam distillation and analyzed by gas chromatography-mass spectrometry (GC-MS). Then the antibacterial and antifungal activities of the essential oils were tested by double dilution and mycelial growth method respectively. RESULTS A total of 37, 48, 38 components from the essential oil of the root, leaf, and peel of this plant were identified. There were 15 components in common, which accounted for 84.05%, 36.83%, 64.46% of the total essential oil in each organ, respectively. The bacteriostatic test showed that the essential oils from different parts of C. praecox (Linn.) Link had different inhibitory effects on the tested strains. CONCLUSION The essential oil of the root contains many bioactive components and has strong antibacterial activity against Gram-positive bacteria as well as pathogenic fungus, which reveals high medicinal value and drug development prospect. |
| Key words: Chimonanthus praecox (Linn.) Link essential oil GC-MS antibacterial activity |
