| 引用本文: | 严长宝,余万鑫,赵妤,戴莉萍,耿福能,万苹,张成桂,巫秀美.康复新液缓解乙酸诱导大鼠急性溃疡性结肠炎及机制研究[J].中国现代应用药学,2019,36(12):1456-1461. |
| YAN Changbao,YU Wanxin,ZHAO Yu,DAI Liping,GENG Funeng,WAN Ping,ZHANG Chenggui,WU Xiumei.Study on the Mechanism and Effect of Kangfuxin on Acute Ulcerative Colitis Induced by Acetic Acid in Rats[J].Chin J Mod Appl Pharm(中国现代应用药学),2019,36(12):1456-1461. |
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| 康复新液缓解乙酸诱导大鼠急性溃疡性结肠炎及机制研究 |
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严长宝1,2, 余万鑫1,3,4, 赵妤1,2, 戴莉萍2,4, 耿福能4,5, 万苹4,6, 张成桂1,3, 巫秀美1,3,4
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1.云南省昆虫生物医药研发重点实验室, 云南 大理 671000;2.大理白族自治州人民医院病理科, 云南 大理 671000;3.药用特种昆虫开发国家地方联合工程研究中心, 云南 大理 671000;4.中国西南药用昆虫及蛛形类资源开发利用协同创新中心, 云南 大理 671000;5.四川好医师药业集团, 成都 610000;6.云南省第一人民医院消化内科, 昆明 650032
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| 摘要: |
| 目的 研究康复新液对乙酸诱导大鼠急性溃疡性结肠炎（ulcerative colitis，UC）细胞因子的影响，并初步探讨其作用机制。方法 采用乙酸灌肠建立急性UC大鼠模型，除正常对照组及模型对照组外，其余各组分别灌肠给予结肠宁、康复新液2.50，1.25，0.625 g·kg-1，测定疾病活动指数（disease activity index，DAI）、结肠黏膜损伤指数（colon mucosa damage index，CMDI）及病理组织学评分（histopathological score，HS），采用酶联免疫吸附法检测大鼠血清中白介素-6（interleukin-6，IL-6）、C反应蛋白（C-reactive protein，CRP）、一氧化氮合酶（nitric oxide synthase，iNOS）以及结肠组织中表皮生长因子（epidermal growth factor，EGF）、环氧合酶-2（cyclooxygenase-2，COX-2）、髓过氧化物酶（myeloperoxidase，MPO）、iNOS的含量。结果 与模型对照组相比，结肠宁及康复新液高剂量能显著降低UC大鼠IL-6、CRP、iNOS、COX-2、MPO表达，升高EGF的表达水平（P<0.01），改善组织病变情况。结论 康复新液对乙酸诱导的UC有治疗作用，作用机制可能与下调大鼠体内炎症因子的表达量，上调EGF的表达量有关。 |
| 关键词: 康复新液 急性溃疡性结肠炎 作用机制 |
| DOI：10.13748/j.cnki.issn1007-7693.2019.12.002 |
| 分类号:R285.5 |
| 基金项目:国家自然科学基金项目（81660605，81860742，81860765）；云南省地方本科高校（部分）基础研究联合专项（2017FH001-108）；药用特种昆虫开发国家地方联合工程研究中心创新能力建设项目（云发改高技[2015]395）；云南省昆虫生物医药研发重点实验室专项经费（2015DG030）；云南省2011协同创新中心项目（[2012]25）；云南省教育厅科学研究基金产业化培育项目（2016CYH15）；云南省创新团队（2018HC006） |
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| Study on the Mechanism and Effect of Kangfuxin on Acute Ulcerative Colitis Induced by Acetic Acid in Rats |
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YAN Changbao1,2, YU Wanxin1,3,4, ZHAO Yu1,2, DAI Liping2,4, GENG Funeng4,5, WAN Ping4,6, ZHANG Chenggui1,3, WU Xiumei1,3,4
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1.Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R & D, Dali 671000, China;2.Department of Pathology, Dali Bai Autonomous Prefecture People's Hospital, Dali 671000, China;3.National and Local Joint Engineering Research Center for the Development of Special Medicinal Insects, Dali 671000, China;4.Chinese Southwest 2011 Collaborative Innovtion Center for Entomoceutics, Dali 671000, China;5.Good Doctor of Sichuan Pharmaceutical Group, Chengdu 610000, China;6.Department of Gastroenterology, The First People's Hospital of Yunnan Province, Kunming 650032, China
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| Abstract: |
| OBJECTIVE To study the effect of Kangfuxin on cytokines of acute ulcerative colitis(UC) induced by acetic acid in rats and explore its mechanism. METHODS The acute UC rat model was established by acetic acid enema. Except the normal control group and model control group, the other groups were gavaged respectively by Jiechangning, Kangfuxin 2.50, 1.25, 0.625 g·kg-1. The disease activity index(DAI), colonic mucosa damage index(CMDI) and histopathological score(HS) were detected, interleukin-6(IL-6), C-reactive protein(CRP), nitric oxide synthase(iNOS) in serum and content of EGF, COX-2, MPO, iNOS in colon were determined by enzyme-linked immunosorbent method. RESULTS Compared with the model control group, Jiechangning and Kangfuxin with high dose could significantly reduce the expression level of IL-6, CRP, iNOS, COX-2, MPO and rise EGF in UC rats(P<0.01), and improve the pathological conditions of tissues. CONCLUSION Kangfuxin has therapeutic effect on acetic acid induced acute UC, and its mechanism may be related to down-regulating the expression of inflammatory factors and up-regulating the expression of EGF. |
| Key words: Kangfuxin acute ulcerative colitis mechanism |
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