| 引用本文: | 李进,陈佳丽,马月光,徐良辉.HPLC测定三棱入血成分含量[J].中国现代应用药学,2019,36(13):1665-1668. |
| LI Jin,CHEN Jiali,MA Yueguang,XU Lianghui.Determination of Components of Sparganii Rhizoma Absorbed into Blood by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2019,36(13):1665-1668. |
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| 摘要: |
| 目的 建立同时测定三棱入血成分对羟基苯甲酸、香草酸、阿魏酸和芦丁含量的方法。方法 采用高效液相色谱法，Agilent Eclipse XDB-C18色谱柱（250 mm×4.6 mm，5 μm）；流动相A为0.1%磷酸水溶液，B为甲醇，进行梯度洗脱；流速1.00 mL·min-1；检测波长254 nm；柱温为30℃。结果 4种成分分离度良好，且均在质量浓度0.000 5~0.25 mg·mL-1内有良好的线性关系。各组含药血清样本的精密度RSD<10%，方法回收率>；90%；各组样品稳定性RSD均<10%。表明本实验精密度良好，回收率和稳定性均符合药动学要求。结论 该检测方法能够同时、快速、准确测定三棱4种有效成分在血清中的含量。 |
| 关键词: 三棱 入血成分 高效液相色谱法 含量测定 |
| DOI：10.13748/j.cnki.issn1007-7693.2019.13.013 |
| 分类号:R917.101 |
| 基金项目:浙江省中医药科技计划项目（2015ZA135）；杭州市卫生科技计划项目（2016A07） |
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| Determination of Components of Sparganii Rhizoma Absorbed into Blood by HPLC |
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LI Jin1, CHEN Jiali2, MA Yueguang2, XU Lianghui2
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1.First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China;2.Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou 310006, China
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| Abstract: |
| OBJECTIVE To establish a method for simultaneous determination of phydroxybenzoic acid, vanillic acid, ferulic acid and rutin absorbed into blood of sparganii Rhizoma. METHODS The analysis was carried out by HPLC, chromatographic column:Agilent Eclipse XDB-C18(250 mm×4.6 mm, 5 μm); the mobile phase A was 0.1% phosphoric acid aqueous and solution B was methanol, and the gradient elution HPLC method was used; the flow rate was 1.00 mL·min-1; the detection wavelength was 254 nm; the column temperature was 30℃. RESULTS The four components showed good separation and good linear relationship in the concentration range of 0.000 5-0.25 mg·mL-1. The precision of serum sample in each group was good(RSD<10%), the recovery rate was >90%, and the stability of each group was good(RSD<10%), which satisfied the requirements of pharmacokinetics. CONCLUSION The method can determine the four active contents of sparganii Rhizoma in serum simultaneously, accurately and rapidly. |
| Key words: sparganii Rhizoma components absorbed into blood HPLC content determination |
