| 引用本文: | 马天红,夏道宗,盛涛,张晓熙,郭璐.2种急性痛风性关节炎小鼠造模方法比较[J].中国现代应用药学,2019,36(8):916-919. |
| MA Tianhong,XIA Daozong,SHENG Tao,ZHANG Xiaoxi,GUO Lu.Comparison of Two Acute Gouty Arthritis Mouse Models[J].Chin J Mod Appl Pharm(中国现代应用药学),2019,36(8):916-919. |
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| 摘要: |
| 目的 比较2种制备急性痛风性关节炎小鼠模型的方法。方法 将18只C57BL/6 ♂小鼠随机分3组，分别采用踝关节腔和足垫注射50 mg·mL-1尿酸钠混悬液25 μL的造模法复制急性痛风性关节炎模型，空白组注射0.9%氯化钠注射液。比较3组小鼠体质量、足肿胀度，ELISA测定踝关节液和血清中的IL-1β表达，并进行组织形态学观察。结果 各组造模前后体质量无统计学差异；踝关节组关节液中IL-1β表达最高，与足垫组和空白组比较具有统计学意义（P<0.05或P<0.01）；且炎症细胞浸润的顺序为踝关节组 > 足垫组 > 空白组。结论 采用踝关节造模法，取踝关节液作为ELISA样本，有利于急性痛风性关节炎的动物实验研究。 |
| 关键词: 痛风性关节炎 动物模型 炎症因子 |
| DOI：10.13748/j.cnki.issn1007-7693.2019.08.004 |
| 分类号:R965.1 |
| 基金项目:国家自然科学基金项目（81673656，81102861）；浙江省中医药科技计划项目（2015ZA062）；浙江省新苗人才计划项目（2017R410021） |
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| Comparison of Two Acute Gouty Arthritis Mouse Models |
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MA Tianhong1,2, XIA Daozong1, SHENG Tao2, ZHANG Xiaoxi1, GUO Lu1
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1.College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China;2.Jiaxing Affiliated Hospital of Zhejiang Chinese Medical University, Jiaxing 314001, China
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| Abstract: |
| OBJECTIVE To compare two methods for preparing mouse models of acute gouty arthritis. METHODS Eighteen C57BL/6 male mice were divided into 3 groups randomly, building acute gouty arthritis model mice with 25 μL 50 mg·mL-1 monosodium urate injection into ankle joint cavity and toe pad respectively, blank group using 0.9% NaCl instead. Weight and foot swelling were compared, as well as expression of IL-1β in ankle fluid and serum. Furthermore, observed the histomorphology in each group. RESULTS There was no statistical difference in weight before or after modeling. The highest expression of IL-1β was found in the articular fluid of the ankle joint group, which was statically significant compared with the foot pad group and blank group(P<0.05 or P<0.01). The intensity of inflammatory cell infiltration was ankle joint group > toe pad group > blank group. CONCLUSION Using ankle joint modeling method and taking ankle joint fluid as ELISA sample is conducive to the animal experimental study of acute gouty arthritis. |
| Key words: gouty arthritis animal model inflammatory cytokines |
