| 引用本文: | 秦斌,王炳志,闫研,王铁杰,殷果,许稳健,严义勇.基于荧光纳米颗粒免疫层析技术的毒死蜱快筛试纸的研制[J].中国现代应用药学,2019,36(11):1348-1351. |
| QIN Bin,WANG Bingzhi,YAN Yan,WANG Tiejie,YIN Guo,XU Wenjian,YAN Yiyong.Development of Chlorpyrifos Test Strip Base on Bifunctional Fluorescent Nanoparticles Immune Chromatography Technology[J].Chin J Mod Appl Pharm(中国现代应用药学),2019,36(11):1348-1351. |
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| 基于荧光纳米颗粒免疫层析技术的毒死蜱快筛试纸的研制 |
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秦斌1,2, 王炳志3, 闫研1,2, 王铁杰1,2, 殷果1,2, 许稳健3, 严义勇3
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1.深圳市药品检验研究院, 深圳 518057;2.深圳药品质量标准研究重点实验室, 深圳 518057;3.深圳市易瑞生物技术股份有限公司, 深圳 518101
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| 摘要: |
| 目的 研制快速检测中药材中毒死蜱的快筛试纸。方法 采用免疫层析技术，以CdTe@SiO2荧光纳米颗粒为标记物制备毒死蜱单抗-荧光纳米颗粒偶联物，以竞争抑制模式制备毒死蜱荧光免疫层析试纸条。结果 该试纸条对中药中毒死蜱的检出限为25 ng·g-1。从开始制样到检测出结果仅需15~20 min。结论 该法操作简便，具有较高的灵敏度和特异性，可广泛应用于中药材中毒死蜱残留的现场筛查和检测。 |
| 关键词: 双功能荧光纳米颗粒 毒死蜱 试纸条 |
| DOI：10.13748/j.cnki.issn1007-7693.2019.11.007 |
| 分类号:R917.101 |
| 基金项目:广东省科技计划项目（2015A030401018，2016A040403022） |
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| Development of Chlorpyrifos Test Strip Base on Bifunctional Fluorescent Nanoparticles Immune Chromatography Technology |
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QIN Bin1,2, WANG Bingzhi3, YAN Yan1,2, WANG Tiejie1,2, YIN Guo1,2, XU Wenjian3, YAN Yiyong3
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1.Shenzhen Institute for Drug Control, Shenzhen 518057, China;2.Shenzhen Key Laboratory of Drug Quality Standard Research, Shenzhen 518057, China;3.Shenzhen Bioeasy Biotechnology Co., Ltd., Shenzhen 518101, China
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| Abstract: |
| OBJECTIVE To make chlorpyrifos test strip for on-site rapid screening and identification of chlorpyrifos in traditional Chinese medicine. METHODS CdTe@SiO2 fluorescent nanoparticle conjugated covalently with monoclonal antibody against chlorpyrifos was employed in the development of lateral flow strip assay by using the immunofluorescence technique. RESULTS This rapid test strip had a detection limit as low as 0.25 ng·kg-1. It was easy of use and only takes 15-20 min to finish the test. CONCLUSION It is very simple in operation, high in sensitivity and specificity, and can be widely used in Chinese medicine screening and detection of chlorpyrifos residue. |
| Key words: bifunctional fluorescent nanoparticles chlorpyrifos lateral flow strip |
