小檗胺对肝癌SMMC-7721细胞侵袭转移的影响

余彬彬; 钱金娥; 曹剑波<sup>*</sup>

引用本文:	余彬彬,钱金娥,曹剑波.小檗胺对肝癌SMMC-7721细胞侵袭转移的影响[J].中国现代应用药学,2018,35(12):1771-1775.
	YU Binbin,QIAN Jin'e,CAO Jianbo.Effect of Berbamine on Migration and Invasion of Human Liver Cancer SMMC-7721 Cells[J].Chin J Mod Appl Pharm(中国现代应用药学),2018,35(12):1771-1775.

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小檗胺对肝癌SMMC-7721细胞侵袭转移的影响
余彬彬^1,2, 钱金娥¹, 曹剑波¹
1.苏州大学附属张家港医院药学部, 江苏苏州 215600;2.苏州大学附属张家港医院转化医学中心, 江苏苏州 215600

摘要:

目的研究小檗胺对人肝癌SMMC-7721细胞侵袭转移作用的影响及其可能的机制。方法在体外用不同浓度小檗胺处理肝癌SMMC-7721细胞，采用MTT法检测细胞活力的变化；采用细胞划痕试验、Transwell迁移和侵袭试验检测肿瘤细胞侵袭转移能力；采用Western blot法检测细胞上皮间质转化相关蛋白E-cadherin和Vimentin的表达。结果 MTT结果显示浓度<20 μmol·L^-1的小檗胺对SMMC-7721细胞存活率无显著影响。划痕试验和Transwell结果显示，小檗胺呈浓度依赖性抑制肝癌SMMC-7721细胞迁移和侵袭过程。Western blot结果表明，小檗胺显著增强SMMC-7721细胞中E-cadherin表达，而减弱Vimentin表达。结论人肝癌SMMC-7721细胞侵袭转移能力可被小檗胺抑制，其机制可能与其调控上皮间质转化相关蛋白的表达有关。

关键词: 肝癌小檗胺 SMMC-7721细胞侵袭转移上皮间质转化

DOI：10.13748/j.cnki.issn1007-7693.2018.12.004

分类号:R285.5

基金项目:苏州市科技计划项目（SYSD2017160）

Effect of Berbamine on Migration and Invasion of Human Liver Cancer SMMC-7721 Cells

YU Binbin^1,2, QIAN Jin'e¹, CAO Jianbo¹

1.The Affliated Zhangjiagang Hospital of Soochow University, Department of Pharmacy, Suzhou 215600, China;2.The Affliated Zhangjiagang Hospital of Soochow University, Center of Translational Medicine, Suzhou 215600, China

Abstract:

OBJECTIVE To study the effect and possible mechanism of berbamine on migration and invasion of human liver cancer SMMC-7721 cells. METHODS After SMMC-7721 cells were incubated with different concentration berbamine in vitro. The cell survival rate was assessed by MTT assay. The effect of berbamine on the migration and invasion of SMMC-7721 cells were detected by wound healing and transwell assays. The expression of E-cadherin and Vimentin protein involved in epithelial mesenchymal transition (EMT) was determined by Western blot assay. RESULTS MTT results showed berbamine exhibited no effect on SMMC-7721 cell survival rate in a range of concentrations. Both wound healing and transwell results showed that berbamine inhibited the migration and invasion of SMMC-7721 cells in a concentration-dependent manner. The Western blot assay confirmed that berbamine could decrease the expression of Vimentin, and increase the expression of E-cadherin. CONCLUSION Berbamine could influence the SMMC-7721 cells migration and invasion, which might be associated with the regulation of the expression of proteins involved in EMT.

Key words: hepatocellular carcinoma berbamine SMMC-7721 cell migration invasion epithelial mesenchymal transition