| 引用本文: | 罗威,彭晓国,叶小弟,方明春,程敏,缪云萍,陈爱瑛,董文彬,郑高利.乌灵菌发酵液抗辐射功能研究[J].中国现代应用药学,2019,36(6):675-681. |
| LUO Wei,PENG Xiaoguo,YE Xiaodi,FANG Mingchun,CHENG Min,MIAO Yunping,CHEN Aiying,DONG Wenbin,ZHENG Gaoli.Research on Radioprotective Activity of Wuling Bacteria Fermentation Bulk[J].Chin J Mod Appl Pharm(中国现代应用药学),2019,36(6):675-681. |
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| 乌灵菌发酵液抗辐射功能研究 |
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罗威1, 彭晓国2, 叶小弟3, 方明春2, 程敏3, 缪云萍3, 陈爱瑛3, 董文彬3, 郑高利1,3
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1.浙江中医药大学药学院, 杭州 310053;2.浙江佐力药业股份有限公司, 杭州 310012;3.浙江省医学科学院, 杭州 310007
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| 摘要: |
| 目的 研究乌灵菌发酵液对小鼠抗辐射功能的影响。方法 通过迟发型超敏反应（delayed type hypersensitivity，DTH）、脾淋巴细胞增殖转化试验、血清溶血素和凝集素水平检测、碳粒廓清试验观察乌灵菌发酵液对免疫功能的影响；通过检测辐射损伤小鼠外周血液学指标、骨髓有核细胞数、骨髓细胞微核率与血清及脏器中的脂质过氧化产物丙二醛（MDA）含量观察乌灵菌发酵液对氧化应激与造血功能的影响。结果 与正常组相比，乌灵菌发酵液3，0.6 g·kg-1剂量组DTH反应明显增强（P<0.01），脾淋巴细胞增殖转化功能、血清溶血素、碳粒廓清指数及胸腺系数显著提高（P<0.05或P<0.01）；与模型组相比，给药组骨髓有核细胞数与骨髓细胞微核率无明显差异，血清与心脏MDA含量显著降低（P<0.05或P<0.01），白细胞数量显著增加（P<0.01），结论 乌灵菌发酵液可能通过增强机体免疫调节能力与造血功能来发挥抗辐射功效。 |
| 关键词: 乌灵菌 发酵液 抗辐射 |
| DOI：10.13748/j.cnki.issn1007-7693.2019.06.006 |
| 分类号:R284.1 |
| 基金项目:浙江省医药卫生科技计划项目（2016155960） |
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| Research on Radioprotective Activity of Wuling Bacteria Fermentation Bulk |
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LUO Wei1, PENG Xiaoguo2, YE Xiaodi3, FANG Mingchun2, CHENG Min3, MIAO Yunping3, CHEN Aiying3, DONG Wenbin3, ZHENG Gaoli1,3
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1.College of Pharmacy, Zhejiang Chinese Medical University, Hangzhou 310053, China;2.Jolly Pharmaceutical, Hangzhou 310012, China;3.Zhejiang Academy of Medical Sciences, Hangzhou 310007, China
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| Abstract: |
| OBJECTIVE To investigate the radioprotective effect of Wuling bacteria fermentation bulk in mice. METHODS The delayed type hypersensitivity(DTH), proliferation and transformation of spleen lymphocytes, the level of serum hemolysin and lectin, and the carbon clearance capability were measured respectively in mice to determine the immune function. In addition, the hematological indexes, bone marrow cells, micronucleus rate of bone marrow cells and lipid peroxide MDA content in model mice with radiation injury were detected to determine the oxidative stress and hematopoietic function. RESULTS The results showed that, compared with the normal group, Wuling bacteria fermentation bulk at dose of 3 g·kg-1 and 0.6 g·kg-1 enhanced the DTH intensity(P<0.01), promoted spleen lymphocytes proliferation and increased clearance index, thymus index and HC50 value(P<0.05 or P<0.01). Compared with the model group, the number of bone marrow nucleated cell and micronucleus rate of bone marrow cells in the Wuling bacteria fermentation bulk-treated groups showed no significant difference, but the content of MDA in serum and heart decreased significantly (P<0.05 or P<0.01), white blood cells increased obviously(P<0.01). CONCLUSION Wuling bacteria fermentation bulk could effectively regulate the radioprotective effect in mice by enhancing the immune and hematopoietic function. |
| Key words: Wuling bacteria fermentation bulk radioprotective |
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