| 引用本文: | 童静玲,朱让腾,罗利飞.珍菊降压片在大鼠体内药动学研究[J].中国现代应用药学,2019,36(4):451-455. |
| TONG Jingling,ZHU Rangteng,LUO Lifei.Pharmacokinetic Study of Zhenju Jiangya Pill in Rats[J].Chin J Mod Appl Pharm(中国现代应用药学),2019,36(4):451-455. |
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| 摘要: |
| 目的 建立同时测定大鼠血浆中芦丁和氢氯噻嗪的LC-MS/MS方法，并研究珍菊降压片在大鼠体内的药动学。方法 采用蛋白质沉淀法处理血浆样品，色谱柱为Pntulips BP-C18柱（2.1 mm×50 mm，5μm），流动相为乙腈-水梯度洗脱，柱温为30℃；流速为0.45 mL·min-1。采用电喷雾离子源，选择离子反应监测模式。采用DAS 2.0软件计算药动学参数。结果 方法学验证结果表明内源性杂质不干扰待测物和内标的测定，芦丁和氢氯噻嗪的线性范围分别为5~1 000 ng·mL-1（r2=0.997 1）和2.5~500 ng·mL-1（r2=0.995 8）。芦丁和氢氯噻嗪药动学参数：AUC（0-t）为（107 157.31±38 056.63），（130 387.28±46 306.69）ng·mL-1·min-1；T1/2z为（108.65±20.95），（240.86±46.44）min；Tmax为（34.25±16.34），（120.00±0.00）min；Cmax为（683.44±254.03），（368.45±136.95）ng·mL-1。结论 该方法准确度、精密度、回收率和基质效应均符合生物基质样品测试要求，适用于珍菊降压片在大鼠体内的药动学研究。 |
| 关键词: 珍菊降压片 芦丁 氢氯噻嗪 药动学 大鼠 |
| DOI：10.13748/j.cnki.issn1007-7693.2019.04.014 |
| 分类号:R917.101 |
| 基金项目:浙江省中医药科学研究基金项目（2015ZB131）；台州市市级科技计划项目（14SF103）；恩泽集团课题（17EZB8） |
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| Pharmacokinetic Study of Zhenju Jiangya Pill in Rats |
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TONG Jingling, ZHU Rangteng, LUO Lifei
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Enze Hospital, Taizhou Enze Medical Center, Taizhou 318050, China
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| Abstract: |
| OBJECTIVE To establish a LC-MS/MS method for the simultaneous determination of rutin and hydrochlorothiazide in rat plasma and to study the pharmacokinetics of Zhenju Jiangya Pill in rats. METHODS The plasma sample was handled by protein precipitation. The column was Pntulips BP-C18 column(2.1 mm×50 mm, 5 μm) with the mobile phase of acetonitrile and water by gradient elution at 0.45 mL·min-1. The column temperature was 30℃. The electrospray ion source and the ion reaction monitoring mode were used. DAS 2.0 software was used to calculate the pharmacokinetic parameters. RESULTS Methodological verification results showed that the endogenous impurities did not interfere with the determination of the analytes and internal standard. The linear ranges of rutin and hydrochlorothiazide were 5-1 000 ng·mL-1(r2=0.997 1) and 2.5-500 ng·mL-1(r2=0.995 8), respectively. The pharmacokinetic parameters of rutin and hydrochlorothiazide were as follows:AUC(0-t) were (107 157.31±38 056.63), (130 387.28±46 306.69)ng·mL-1·min, T1/2z were (108.65±20.95), (240.86±46.44)min, Tmax were (34.25±16.34), (120.00±0.00)min, Cmax were (683.44±254.03), (368.45±136.95)ng·mL-1. CONCLUSION The precision, accuracy, mean recoveries and the matrix effects were satisfied with the requirements of biological sample measurement. The method was successfully applied to the pharmacokinetic study of Zhenju Jiangya Pill in rats. |
| Key words: Zhenju Jiangya Pill rutin hydrochlorothiazide pharmacokinetic rats |
