| 引用本文: | 李博,胡秋侠,谭锦辉,吴系美,佘若男,胡建云.类风湿关节炎中miR-146a多态性与临床表现及血清中肿瘤坏死因子α水平之间的相关性[J].中国现代应用药学,2019,36(4):471-474. |
| LI Bo,HU Qiuxia,TAN Jinhui,WU Ximei,SHE Ruonan,HU Jianyun.Mir-146a Rs2910164 Polymorphism Correlates with Extraarticular Manifestations and the Serum TNF-α Level in Patients with Rheumatoid Arthritis[J].Chin J Mod Appl Pharm(中国现代应用药学),2019,36(4):471-474. |
|
| 摘要: |
| 目的 探讨类风湿关节炎（rheumatoid arthritis，RA）中miR-146a上rs2910164位点单核苷酸多态性（SNP）与患者临床表现及血清中肿瘤坏死因子α（tumor necrosis factor-α，TNF-α）表达水平之间的相关性。方法 以高分辨率熔解（high-resolution melting，HRM）法对126例RA患者进行miR-146a上rs2910164位点的SNP分型，并分析其与RA患者病情活动性、临床表现及患者血清中TNF-α表达水平之间是否有关。结果 RA患者中miR-146a上rs2910164位点G等位基因频率为30.6%，C等位基因频率为69.4%，G/G型纯合子患者的比例为12.7%，C/C型纯合子患者的比例为51.6%，G/C型杂合子患者的比例为35.7%。未发现miR-146a上rs2910164位点的不同基因型组之间的病情活动性有显著差异，但具有G/C基因型的RA患者出现关节外表现的比例显著增高（P<0.05），具有G/C基因型的RA患者血清中细胞因子TNF-α表达水平显著高于具有G/G基因型[（5.41±0.69）vs（3.63±0.37），P<0.05]和C/C基因型[（5.41±0.69）vs（3.27±0.82），P<0.05]的RA患者。结论 miR-146a上rs2910164位点SNP与RA病情活动性无明显相关性，但G/C基因型与RA特定的临床表现及血清中TNF-α表达水平有关，提示miR-146a可能在RA发病中可能具有一定的作用。 |
| 关键词: miR-146a 类风湿关节炎 单核苷酸多态性 肿瘤坏死因子α |
| DOI：10.13748/j.cnki.issn1007-7693.2019.04.018 |
| 分类号:R965.2 |
| 基金项目:广东省医学科研基金立项课题资助（A2013611）；深圳市卫生计生系统科研项目（201501051，201601060）；深圳市龙华区科技创新资金项目（20170919A1030357） |
|
| Mir-146a Rs2910164 Polymorphism Correlates with Extraarticular Manifestations and the Serum TNF-α Level in Patients with Rheumatoid Arthritis |
|
LI Bo1, HU Qiuxia2, TAN Jinhui1, WU Ximei1, SHE Ruonan1, HU Jianyun2
|
|
1.People's Hospital of Longhua District Shenzhen, Department of Rheumatology, Shenzhen 518109, China;2.People's Hospital of Longhua District Shenzhen, Department of Rheumatology & Nephrology, Shenzhen 518109, China
|
| Abstract: |
| OBJECTIVE To investigate the single nucleotide polymorphisms(SNP) of the microRNA-146a (miR-146a) in rheumatoid arthritis(RA), and whether it is associated with clinical manifestations and the serum tumor necrosis factor-α(TNF-α) level in patients with RA. METHODS The SNP of miR-146a rs2910164 polymorphism was detected by high-resolution melting method in 126 patients with RA, and the serum TNF-α level was detected by enzyme linked immunosorbent assay(ELISA). The correlations of miR-146a rs2910164 polymorphism with clinical manifestations and the serum TNF-α level were also analyzed. RESULTS The percent of RA patients with G/C, G/G and C/C genotype was 35.7%, 12.7% and 51.6% respectively. It was not found that any significant association of miR-146a rs2910164 polymorphism with disease activity in RA. However, the percent of RA patients with extraarticular manifestations was higher in miR-146a rs2910164 G/C genotype than in RA patients with other genotypes (P<0.05), and the serum level of TNF-α was higher in miR-146a rs2910164 G/C genotype than in RA patients with other genotypes(P<0.05). CONCLUSION It suggests that miR-146a rs2910164 polymorphism might correlate with special clinical manifestations and higher levels of serum level of TNF-α in RA, which indicate that miR-146a may play an important role in pathogenesis of RA. |
| Key words: miR-146a rheumatoid arthritis single nucleotide polymorphisms tumor necrosis factor-α(TNF-α) |
